Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens
Comparative evaluation of three commercial systems for nucleic acid extraction from urine specimens
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DOI：
10.1128/jcm.43.9.4830-4833.2005
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发表时间：
2005-09-01
影响因子：
9.4
通讯作者：
Procop, GW
中科院分区：
文献类型：
--
作者：
Tang, YW;Sefers, SE;Procop, GW
一个核酸提取系统，可以处理少量的样本，测试周转时间短，动手时间短，是紧急测试的理想选择。我们在三个系统上进行了比较验证：Magna Pure Compact System(紧凑型)、NucliSens mini MAG提取仪器(Mini MAG)和BioRobot EZ1系统(EZ1)。总共使用了75份提交用于多瘤病毒BK病毒检测的尿样。分别在75(100%)、75(100%)和72(96%)的核酸提取液中检测到人β-肌动蛋白基因。在三个系统中，微型MAG产生的核酸数量最多，精确度最高。三种提取体系制备的核酸提取液检测BKV的符合率均为100%。当运行一整块试件时，微型MAG的动手时间和测试周转时间分别为105.7和121.1分钟，EZ1为6.1min和22.6min，Comp为7.4min和33.7min。EZ1和紧凑型系统正确地处理了自动核酸提取，为零星但紧急的样本检测提供了一个很好的解决方案。微型MAG产生了最高数量的核酸，这表明该系统将是包含少量感兴趣微生物的标本的最佳选择。
A nucleic acid extraction system that can handle small numbers of specimens with a short test turnaround time and short hands-on time is desirable for emergent testing. We performed a comparative validation on three systems: the MagNA Pure compact system (Compact), the NucliSens miniMAG extraction instrument (miniMAG), and the BioRobot EZ1 system (EZ1). A total of 75 urine specimens submitted for polyomavirus BK virus detection were used. The human beta-actin gene was detected on 75 (100%), 75 (100%), and 72 (96%) nucleic acid extracts prepared by the miniMAG, EZ1, and Compact, respectively. The miniMAG produced the highest quantity of nucleic acids and the best precision among the three systems. The agreement rate was 100% for BKV detection on nucleic acid extracts prepared by the three extraction systems. When a full panel of specimens was run, the hands-on time and test turnaround time were 105.7 and 121.1 min for miniMAG, 6.1 and 22.6 min for EZ1, and 7.4 and 33.7 min for Compact, respectively. The EZ1 and Compact systems processed automatic nucleic acid extraction properly, providing a good solution to the need for sporadic but emergent specimen detection. The miniMAG yielded the highest quantity of nucleic acids, suggesting that this system would be the best for specimens containing a low number of microorganisms of interest.