Complete genome analysis of RFLP 184 isolates of porcine reproductive and respiratory syndrome virus
Complete genome analysis of RFLP 184 isolates of porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.virusres.2006.06.003
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发表时间：
2006-12-01
期刊：
影响因子：
5
通讯作者：
Faaberg, Kay S.
中科院分区：
文献类型：
--
作者：
Han, Jun;Wang, Yue;Faaberg, Kay S.
对最近出现的猪繁殖与呼吸综合征病毒 (PRRSV) 强毒分离株的两个全长基因组进行了测序，并与其他 PRRSV 毒株进行了比较。结果显示，这两个北美谱系分离株（名为 MN 184）代表了迄今为止测序的最短的 PRRSV 基因组，核苷酸长度为 15019 个碱基。遗传分析表明，这两个分离株并不相同，并且分别与原型北美菌株 VR-2332 和欧洲菌株 Lelystad 具有约 87% 和 59% 的核苷酸同一性。鉴定出三个相当可变的区域，对应于推定的 nsp1 beta、推定的 nsp2 和 ORF5。 Nsp2 是变异最大的区域，与其他已测序的类似北美 PRRSV nsp2 蛋白仅具有 66-70% 的氨基酸相似性。进一步研究表明，与菌株VR-2332的nsp2蛋白相比，MN 184分离株的nsp2蛋白含有3个不连续缺失，其大小分别为111、1和19个氨基酸，对应于菌株VR-2332的位置324-434、486和505-523。结果表明，通过反向遗传学进行 nsp2 修饰来靶向操作 PRRSV 可能会产生用于疫苗开发的有希望的载体，正如最近所证明的那样 [Han, J., Faaberg, K.S., Wang, Y., Liu, H., 2005。基于 RFLP 184 分离株中观察到的缺失，PRRSV 株 VR-2332 感染性克隆的非结构蛋白 2 突变体是 可行的。见：PRRS 国际研讨会论文集，卷。 8，密苏里州圣路易斯]。 (c) 2006 年，Elsevier B.V. 出版
Two full-length genomes of recently emerged virulent isolates of porcine reproductive and respiratory syndrome virus (PRRSV) were sequenced and compared to other PRRSV strains. The results revealed that these two isolates (named MN 184), of North American lineage, represented the shortest PRRSV genomes sequenced to date with a nucleotide length of 15019 bases. Genetic analysis demonstrated that the two isolates were not identical and shared approximately 87 and 59% nucleotide identity with prototype North American strain VR-2332 and European strain Lelystad, respectively. Three quite variable regions were identified, corresponding to putative nsp1 beta, putative nsp2 and ORF5. Nsp2, the most variable region, shared only 66-70% amino acid similarity to other sequenced North American-like PRRSV nsp2 proteins. Further study revealed that the nsp2 protein of the MN 184 isolates contained three discontinuous deletions when compared to strain VR-2332 nsp2 protein, with the sizes of 111, 1, and 19 amino acids corresponding to strain VR-2332 positions 324-434, 486 and 505-523, respectively. The results suggest that targeted manipulation of PRRSV through nsp2 modification by reverse genetics may yield promising vectors for vaccine development, as has been recently demonstrated [Han, J., Faaberg, K.S., Wang, Y., Liu, H., 2005. Non-structural protein 2 mutants of PRRSV strain VR-2332 infectious clone based on deletions seen in RFLP 184 isolates are viable. In: PRRS International Symposium Proceedings, vol. 8, Saint Louis, MO]. (c) 2006 Published by Elsevier B.V.