Human parainfluenza virus type 2 V protein inhibits induction of tetherin
Human parainfluenza virus type 2 V protein inhibits induction of tetherin
复制标题
人副流感病毒 2 型 V 蛋白抑制系链蛋白的诱导
DOI：
10.1007/s00430-017-0508-z
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Nishio Machiko
中科院分区：
文献类型：
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作者：
Ohta Keisuke;Matsumoto Yusuke;Yumine Natsuko;Nishio Machiko
Tetherin是一种抗病毒因子，其通过将病毒粒子束缚到细胞表面来限制病毒出芽。人副流感病毒2型（hPIV-2）V蛋白减少HeLa细胞中的细胞表面系链蛋白，HeLa细胞组成型表达内源性系链蛋白。然而，hPIV-2 V蛋白在系链蛋白诱导中的作用仍不清楚。在这里，我们研究了hPIV-2感染本身是否在没有基础tetherin表达的HEK 293细胞中诱导tetherin。与甲型流感病毒（IAV）感染不同，hPIV-2感染既不诱导系链蛋白mRNA也不诱导蛋白表达。相比之下，通过感染携带V突变体的rPIV-2观察到了稳健的拴系蛋白诱导，其中对减少细胞表面拴系蛋白很重要的三个Trp残基（W178 H/W182 E/W192 A）或Cys残基（C209/211/214 A）发生突变。hPIV-2感染也抑制IFN-α和IAV感染诱导的Tetherin表达。hPIV-2 V蛋白抑制tetherin的诱导作用，而P蛋白和VW 178 H/W182 E/W192 A蛋白对tetherin的诱导作用无影响。我们的数据共同表明，hPIV-2 V蛋白抑制由几种外部刺激诱导的tetherin表达。
Tetherin is an anti-viral factor that restricts viral budding through tethering virions to the cell surface. The human parainfluenza virus type 2 (hPIV-2) V protein decreases cell surface tetherin in HeLa cells, which constitutively express endogenous tetherin. However, the role of the hPIV-2 V protein in tetherin induction remains unclear. Here, we examined whether hPIV-2 infection itself induces tetherin in HEK293 cells that have no basal expression of tetherin. Unlike influenza A virus (IAV) infection, hPIV-2 infection induced neither tetherin mRNA nor protein expression. In contrast, robust tetherin induction was observed by infection of rPIV-2s carrying V mutants, in which either three Trp residues (W178H/W182E/W192A) or Cys residues (C209/211/214A) that are important for decreasing cell surface tetherin are mutated. hPIV-2 infection also inhibited the induction of tetherin expression by IFN-α and IAV infection. Furthermore, hPIV-2 V protein but not P and VW178H/W182E/W192Asuppressed tetherin induction. Our data collectively suggest that the hPIV-2 V protein inhibits tetherin expression induced by several external stimuli.