Long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis.
Long sequences in the 5' noncoding region of plum pox virus are not necessary for viral infectivity but contribute to viral competitiveness and pathogenesis.
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李痘病毒 5 非编码区的长序列对于病毒感染性不是必需的，但有助于病毒竞争力和发病机制。
DOI：
10.1006/viro.1997.8574
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发表时间：
1997
期刊：
影响因子：
3.7
通讯作者：
Juan Antonio García
中科院分区：
文献类型：
--
作者：
L. Simón;Hui Shan Guo;Juan Antonio García
梅痘马铃薯Y病毒（plumepoxpotyvirus，PPV）5'端非编码区（noncodingregion，5'端31个核苷酸）在马铃薯Y病毒属（Potyvirus）所有成员中高度保守。为了绘制体内感染性所必需的5'非编码区的序列，我们构建了一组嵌套的取代和缺失突变体。虽然我们不能用病毒基因组5 '端35个核苷酸突变的全长PPV转录物感染烟草植物，但位于核苷酸39和145之间的长序列的缺失并没有改变感染率或病毒积累。然而，这些突变体在共接种实验中不能与野生型菌株竞争。感染了PPV突变体，缺乏核苷酸127至145的植物表现出非常轻微的病理学，野生型症状的严重程度后恢复自发的第二个位点突变。
The 5'-terminal 31 nucleotides of the 146-nucleotides-long 5' noncoding region of plum pox potyvirus (PPV) are highly conserved in all the members of the Potyvirus genus. To map the sequences of the 5' noncoding region that are necessary in vivo for infectivity, we have constructed a nested set of substitution and deletion mutants. While we were not able to infect Nicotiana clevelandii plants with full-length PPV transcripts bearing mutations in the 5'-terminal 35 nucleotides of the viral genome, the deletion of long sequences located between nucleotides 39 and 145 did not alter either the rate of infection or viral accumulation. Nevertheless, these mutants were not able to compete with the wild-type strain in coinoculation experiments. Plants infected with a PPV mutant that lacked nucleotides 127 to 145 showed a very mild symptomathology; the wild-type symptom severity was recovered after spontaneous second-site mutations.
豇豆褪绿斑驳病毒RNA3的5端和顺反子间非编码区的大部分对于全身性感染是可有可无的，但会影响病毒竞争力和感染病理学。
影响因子：
3.7
作者：
Pacha,RF;Ahlquist,P
通讯作者：
Ahlquist,P