Variation at phenological candidate genes correlates with timing of dispersal and plumage morph in a sedentary bird of prey
Variation at phenological candidate genes correlates with timing of dispersal and plumage morph in a sedentary bird of prey
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DOI：
10.1111/mec.12493
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发表时间：
2013-11-01
影响因子：
4.9
通讯作者：
Krueger, Oliver
中科院分区：
文献类型：
--
作者：
Chakarov, Nayden;Jonker, Rudy M.;Krueger, Oliver
参与昼夜节律和生物钟保守分子信号传导的多态性基因可能在控制繁殖和扩散的时间方面发挥重要作用，从而影响脊椎动物的健康。然而，相对较少的研究探讨了物候候选基因和行为之间的关联，并且这些研究在一定程度上偏向于特定的分类群体，例如雀形目鸟类和鲑鱼。因此，我们分析了普通鵟（Buteo buteo）调节基因 CLOCK、NPAS2、ADCYAP1 和 CREB1 的外显子和 3 个非翻译区域内的微卫星多态性，鵟是一种多态性猛禽物种，具有三种羽毛形态，这些羽毛形态在关键生活史特征（包括终生繁殖成功率）方面有所不同。与雀形目动物的研究相反，CLOCK 聚谷氨酰胺 (poly-Q) 在 976 只常见秃鹰雏鸟以及其他三种鵟物种中被发现是单态的。此外，没有一个候选基因与羽化日期显着相关，尽管发现中等黑化的雌性平均比浅色或深色形态个体产蛋更早，而且它们的后代携带更长的 ADCYAP1 等位基因。相比之下，所有三个候选基因都解释了幼秃鹰扩散的一项或多项指标（重视概率、扩散时间和扩散距离）的显着变化。我们的研究结果有助于更广泛地研究 CLOCK 多态性的适应性意义，同时也建立在先前记录 ADCYAP1 变异性和迁移时间之间联系的研究的基础上。
Polymorphic genes involved in the conserved molecular signalling of circadian and circannual clocks may play important roles in governing the timing of breeding and dispersal and thereby affect fitness in vertebrates. However, relatively few studies have explored associations between phenological candidate genes and behaviour, and these are somewhat biased towards particular taxonomic groups such as passerine birds and salmonid fish. Consequently, we assayed microsatellite polymorphisms within the exonic and 3 untranslated regions of the regulatory genes CLOCK, NPAS2, ADCYAP1 and CREB1 in the common buzzard (Buteo buteo), a polymorphic raptor species with three plumage morphs that differ in key life history traits including lifetime reproductive success. In contrast to studies of passerines, CLOCK poly-glutamine (poly-Q) was found to be monomorphic in 976 common buzzard nestlings as well as in three other Buteo species. Moreover, none of the candidate genes were significantly associated with fledging dates, although intermediately melanized females were found to lay earlier on average than light or dark morph individuals, and their offspring carried longer ADCYAP1 alleles. In contrast, all three candidate genes explained significant variation in one or more measures of juvenile buzzard dispersal (resighting probability, timing of dispersal and distance dispersed). Our findings contribute towards a broader body of work on the adaptive significance of CLOCK polymorphism, while also building upon previous studies that have documented links between ADCYAP1 variability and the timing of migration.