Identification of a novel cytochrome P450, CYP4X1, with unique localization specific to the brain
Identification of a novel cytochrome P450, CYP4X1, with unique localization specific to the brain
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DOI：
10.1016/s0006-291x(02)00918-x
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发表时间：
2002-08-23
影响因子：
3.1
通讯作者：
Harder, DR
中科院分区：
文献类型：
--
作者：
Bylund, J;Zhang, CY;Harder, DR
用逆转录-聚合酶链反应（RT-PCR）技术从大鼠脑组织中克隆了一种新的细胞色素P450（P450或CYP 4）亚型。核苷酸序列含有一个开放阅读框，编码507个氨基酸。推导的氨基酸序列与大鼠CYP 4亚家族4A、4 B和4F的氨基酸序列具有41-51%的同源性。这种酶被命名为CYP 4X 1。北方印迹分析表明，CYP 4X 1在大脑中高度特异性表达。原位杂交实验表明，CYP 4X 1主要在不同区域的神经元中表达，脑干、海马、皮质和小脑以及血管内皮细胞中。这种新型P450酶的功能尚不清楚，但CYP 4X 1的表达模式表明，CYP 4X 1可能在神经血管功能中发挥作用。CYP 4X 1的催化性质和生理功能目前正在研究中。(C)2002 Elsevier Science（美国）。All rights reserved.
A novel cytochrome P450 (P450 or CYP) isoform belonging to the CYP4 family was cloned with reverse transcription-polymerase chain reaction from rat brain. The nucleotide sequence contained an open reading frame coding for 507 amino acids. The deduced amino acid sequence showed 41-51% identity with that of members of the rat CYP4 subfamilies 4A, 4B, and 4F. The enzyme was designated CYP4X1. Northern blot analysis showed that CYP4X1 is highly and specifically expressed in the brain. In situ hybridization experiments suggest that CYP4X1 is mainly expressed in neurons in different regions, e.g., the brain stem, hippocampus, cortex, and cerebellum as well as in vascular endothelial cells. The function of this novel P450 enzyme is unknown, but the expression pattern of CYP4X1 suggests that it is possible that CYP4X1 plays a role in neurovascular function. The catalytic properties and physiological function of CYP4X1 are currently under investigation. (C) 2002 Elsevier Science (USA). All rights reserved.