SuperSAGE array:: the direct use of 26-base-pair transcript tags in oligonucleotide arrays
SuperSAGE array:: the direct use of 26-base-pair transcript tags in oligonucleotide arrays
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DOI：
10.1038/nmeth882
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发表时间：
2006-06-01
期刊：
影响因子：
48
通讯作者：
Terauchi, Ryohei
中科院分区：
文献类型：
--
作者：
Matsumura, Hideo;Bin Nasir, Khairun Hisam;Terauchi, Ryohei
我们开发了一种新的平台，用于任何真核生物的全基因组基因表达分析，我们称之为SuperSAGE阵列。SuperSAGE阵列是在其上直接合成对应于SuperSAGE标签序列的26-bp寡核苷酸的微阵列。SuperSAGE微阵列结合了SuperSAGE高定量表达分析和高通量微阵列技术的优点。我们通过SuperSAGE阵列对水稻中的1，000个基因（标签）进行了高度可重复的基因表达谱分析。我们还应用这种技术的SuperSAGE确定的表达基因的详细研究，在烟草本塞姆氏，一种生物体，其中足够的基因组序列信息是不可用的。我们建议，SuperSAGE阵列系统代表了一种新的模式，微阵列建设，因为没有基因组或cDNA序列数据是必需的，其准备。
We developed a new platform for genome-wide gene expression analysis in any eukaryotic organism, which we called SuperSAGE array. The SuperSAGE array is a microarray onto which 26-bp oligonucleotides corresponding to SuperSAGE tag sequences are directly synthesized. A SuperSAGE array combines the advantages of the highly quantitative SuperSAGE expression analysis with the high-throughput microarray technology. We demonstrated highly reproducible gene expression profiling by the SuperSAGE array for 1,000 genes ( tags) in rice. We also applied this technology to the detailed study of expressed genes identified by SuperSAGE in Nicotiana benthamiana, an organism for which sufficient genome sequence information is not available. We propose that the SuperSAGE array system represents a new paradigm for microarray construction, as no genomic or cDNA sequence data are required for its preparation.