Molecular characterization of a consistent 4.5-megabase deletion at 4q28 in prostate cancer cells
Molecular characterization of a consistent 4.5-megabase deletion at 4q28 in prostate cancer cells
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DOI：
10.1016/j.cancergencyto.2004.09.010
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发表时间：
2005-05-01
影响因子：
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通讯作者：
Cowell, JK
中科院分区：
文献类型：
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作者：
Matsui, SI;LaDuca, J;Cowell, JK
前列腺细胞系 LNCaP、DU145、PC3 和 22RV 的光谱核型分析表明，结构性染色体重排涉及 4 号染色体的远端长臂。在除 22RV 之外的所有细胞系中，这些都是 4 号和 10 号染色体之间的非相互易位。在 22RV 中，可以看到明显相互的 t(2q;4q)。 4 号染色体易位断点的荧光原位杂交分析表明，缺失与所有易位相关，导致染色体材料的净损失。在 LNCap、DU145 和 22RV 中发现了与 34 类似的 4q28 中的重叠缺失，其定义了大约 4.5 兆碱基对的公共缺失区域。 PC3 中的缺失更靠近 4q，涉及与 q26 区域类似的 4q21。对已发表研究中染色体材料丢失高分辨率定义的荟萃分析表明，至少 50% 的原发性肿瘤可能发生 4q 材料丢失。该分析定义了关键 4q 区域的一系列基因，这些基因可能与前列腺肿瘤的发展相关。 (c) 2005 Elsevier Inc. 保留所有权利。
Spectral karyotyping of prostate cell lines LNCaP, DU145, PC3, and 22RV demonstrated structural chromosome rearrangements involving the distal long arm of chromosome 4. In all but 22RV, these are nonreciprocal translocations between chromosomes 4 and 10. In 22RV, an apparently reciprocal t(2q;4q) is seen. Fluorescence in situ hybridization analysis of the chromosome 4 translocation breakpoints demonstrated that deletions were associated with all of the translocations, resulting in a net loss of chromosome material. Overlapping deletions in 4q28 similar to 34 were seen in LNCap, DU145, and 22RV, which defined an approximately 4.5-megabase pair common region of deletion. The deletion in PC3 was more proximal on 4q, involving the 4q21 similar to q26 region. A meta analysis of high-resolution definition of losses of chromosome material from published studies demonstrates that loss of 4q material may occur in at least 50% of primary tumors. This analysis defines a series of genes in the critical 4q region, which is potentially associated with prostate tumor development. (c) 2005 Elsevier Inc. All rights reserved.