Eyes absent tyrosine phosphatase activity is not required for Drosophila development or survival.
Eyes absent tyrosine phosphatase activity is not required for Drosophila development or survival.
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DOI：
10.1371/journal.pone.0058818
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Mardon G
中科院分区：
文献类型：
--
作者：
Jin M;Jusiak B;Bai Z;Mardon G
缺眼 (Eya) 是一种进化上保守的转录共激活剂和蛋白磷酸酶，可调节后生动物的多个发育过程。果蝇 eya 对于生存以及成虫眼睛的形成是必需的。 Eya 含有酪氨酸磷酸酶结构域，改变假定活性位点残基的突变会导致异位眼诱导、使用 Gal4-UAS 系统的体内遗传拯救和体外磷酸酶测定中的活性大大降低。然而，这些突变尚未在正常发育过程中以正确的内源性 eya 表达水平、时间和模式进行分析。为了研究 Eya 的酪氨酸磷酸酶活性是否在果蝇存活或正常眼睛形成中发挥作用，我们生成了三个 eya 基因组拯救 (eyaGR) 构建体，这些构建体改变了关键活性位点残基，并在体内测试了它们。与之前的研究形成鲜明对比的是，所有 eyaGR 构建体都完全恢复了 eya 无效突变背景中的眼睛形成和活力。我们得出的结论是，Eya 的酪氨酸磷酸酶活性对于果蝇的正常眼睛发育或生存来说不是必需的。我们的研究表明需要重新评估 Eya 的作用机制，并强调在其原生环境中研究基因的重要性。
Eyes absent (Eya) is an evolutionarily conserved transcriptional coactivator and protein phosphatase that regulates multiple developmental processes throughout the metazoans. Drosophila eya is necessary for survival as well as for the formation of the adult eye. Eya contains a tyrosine phosphatase domain, and mutations altering presumptive active-site residues lead to strongly reduced activities in ectopic eye induction, in vivo genetic rescue using the Gal4-UAS system, and in vitro phosphatase assays. However, these mutations have not been analyzed during normal development with the correct levels, timing, and patterns of endogenous eya expression. To investigate whether the tyrosine phosphatase activity of Eya plays a role in Drosophila survival or normal eye formation, we generated three eya genomic rescue (eyaGR) constructs that alter key active-site residues and tested them in vivo. In striking contrast to previous studies, all eyaGR constructs fully restore eye formation as well as viability in an eya null mutant background. We conclude that the tyrosine phosphatase activity of Eya is not required for normal eye development or survival in Drosophila. Our study suggests the need for a re-evaluation of the mechanism of Eya action and underscores the importance of studying genes in their native context.
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影响因子：
8
作者：
Miller, S. J.;Lan, Z. D.;Hardiman, A.;Wu, J.;Kordich, J. J.;Patmore, D. M.;Hegde, R. S.;Cripe, T. P.;Cancelas, J. A.;Collins, M. H.;Ratner, N.
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30.8
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通讯作者：
Francis-Lang, HL