Induced preference for host plant chemicals in the tobacco hornworm: contribution of olfaction and taste
Induced preference for host plant chemicals in the tobacco hornworm: contribution of olfaction and taste
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DOI：
10.1007/s00359-009-0434-7
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发表时间：
2009-06-01
影响因子：
2.1
通讯作者：
Rai, Meelu
中科院分区：
文献类型：
--
作者：
Glendinning, John I.;Foley, Cassidy;Rai, Meelu
许多食草性昆虫诱导对寄主植物的偏爱。最近在Manduca sexta上的研究表明，诱导偏好是通过外周味觉系统对寄主植物叶片中的化学物质进行调节来调节的。我们用人工饲料或土豆叶饲养毛虫，然后测试对土豆叶提取物的嗅觉和味觉调节反应，从而验证了这一假设。首先，我们证实了早先的报道，即食用马铃薯叶可以通过降低对葡萄糖的反应性和增加对叶提取物的反应性来调节外周味觉系统。其次，我们让毛毛虫在用叶提取物处理的圆盘(实验)或单独使用溶剂(对照)之间进行选择。叶部饲养的毛虫不成比例地接近和消耗实验盘，而饮食饲养的毛虫不分青红皂白地接近和消耗两个圆盘。这表明诱导偏好与嗅觉和味觉有关。第三，我们对没有嗅觉或味觉输入的树叶饲养的毛虫进行了选择测试。缺乏嗅觉输入的毛虫不分青红皂白地靠近两个视盘，但选择性地摄食实验视盘。相比之下，缺乏味觉输入的毛虫有选择地接近实验视盘，但不分青红皂白地摄食这两种类型的视盘。我们的结论是，尽管嗅觉帮助毛虫定位土豆的叶子，但最终调节诱导偏好的是“调谐”的味觉反应。
Many herbivorous insects induce preferences for host plants. Recent work in Manduca sexta indicates that induced preferences are mediated by a "tuning" of the peripheral taste system to chemicals within host plant foliage. We tested this hypothesis by rearing caterpillars on artificial diet or potato foliage, and then examining olfactory- and taste-mediated responses to potato foliage extract. First, we confirmed earlier reports that consumption of potato foliage tunes the peripheral taste system by reducing responsiveness to glucose and increasing responsiveness to foliage extract. Second, we offered caterpillars a choice between disks treated with foliage extract (experimental) or solvent alone (control). The foliage-reared caterpillars approached and consumed the experimental disks disproportionately, whereas the diet-reared caterpillars approached and consumed both disks indiscriminately. This indicated that induced preferences involve olfaction and taste. Third, we ran choice tests with foliage-reared caterpillars deprived of either olfactory or gustatory input. Caterpillars lacking olfactory input approached both disks indiscriminately, but fed selectively on experimental disks. In contrast, caterpillars lacking gustatory input approached experimental disks selectively, but fed indiscriminately on both types of disk. We conclude that even though olfaction helps caterpillars locate potato foliage, it is the "tuned" gustatory response that ultimately mediates the induced preference.