REACTIVE MICROGLIA EXPRESS CLASS-I AND CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS IN ALZHEIMERS-DISEASE
REACTIVE MICROGLIA EXPRESS CLASS-I AND CLASS-II MAJOR HISTOCOMPATIBILITY COMPLEX ANTIGENS IN ALZHEIMERS-DISEASE
复制标题
DOI：
10.1016/0006-8993(90)91496-4
复制
发表时间：
1990-07-23
期刊：
影响因子：
2.9
通讯作者：
MCGEER, PL
中科院分区：
文献类型：
--
作者：
TOOYAMA, I;KIMURA, H;MCGEER, PL
本文研究了阿尔茨海默病（Alzheimer's disease，AD）和年龄匹配的正常人脑组织中主要组织相容性复合体（major histocompatibility complex，MHC）Ⅰ类（HLA-A，B，C）和Ⅱ类（HLA-DR）抗原的表达。I类抗原的单克隆抗体对阿尔茨海默氏病灰质和白色物质中的反应性小胶质细胞和血管内皮细胞进行了弱至中度染色。在对照组脑中，captamins也被强烈染色，但在白色物质中只能观察到少数阳性染色的小胶质细胞。在阿尔茨海默病的情况下，HLA-DR阳性反应性小胶质细胞比MHC I类阳性反应性小胶质细胞更多，更强烈的染色，但许多细胞似乎表达两类抗原。来源不明的罕见大细胞也呈I类阳性染色。双重免疫组化证实，MHC I类阳性细胞是一个独立的群体，从胶质细胞酸性蛋白阳性星形胶质细胞。
Expression of major histocompatibility complex (MHC) class I (HLA-A,B,C) and class II (HLA-DR) antigens was studied in postmortem brain tissue of Alzheimer''s disease and age-matched control cases. Monoclonal antibodies to class I antigens stained reactive microglia weakly to moderately and vascular endothelial cells strongly in Alzheimer''s gray and white matter. Capillaries were also stained strongly in control brains, but only a few positively stained microglia could be observed in white matter. In Alzheimer cases, HLA-DR positive reactive microglia were more numerous and more strongly staining than MHC class I positive reactive microglia but many cells appeared to express both classes of antigen. Rare large cells of uncertain origin also stained positively for class I. Double immunostaining established that MHC class I positive cells were a separate population from glial fibrillary acidic-protein-positive astrocytes.