Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae)
Male Selenoprotein F-Like (SPF-L) Influences Female Reproduction and Population Growth in Nilaparvata lugens (Hemiptera: Delphacidae)
复制标题
雄性硒蛋白 F 样 (SPF-L) 影响褐飞虱雌性繁殖和种群增长（半翅目：飞虱科）
DOI：
10.3389/fphys.2019.01196
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发表时间：
2019-09-25
影响因子：
4
通讯作者：
Song, QiSheng
中科院分区：
文献类型：
--
作者：
Ge, LinQuan;Zhou, YongKai;Song, QiSheng
硒蛋白在几乎所有生物体中都具有抗氧化和细胞氧化还原功能。最近的一项研究表明，在褐飞虱雄性附腺（MAG）中存在一种硒蛋白F样（SPF-L），这引起了一个问题，即SPF-L是否与雌性生殖力有关。在这项研究中，发现SPF-L mRNA在处女雄性的内部生殖器官（IRO）中富集，在处女雄性和雌性中也相对稳定地表达，并且饮食dsSPF-L处理导致MAG蛋白和精氨酸含量降低。在未交配的雄性中NlSPF-L的敲低不影响保幼激素（JH）III和蜕皮类固醇滴度，然而，与对照相比，dsSPF-L处理的交配的雄性具有增加的JH III滴度和减少的蜕皮类固醇滴度。与dsSPF-L处理的雄性交配后，雌性伴侣的脂肪体和卵巢可溶性蛋白以及JH III层和卵黄蛋白原（Vg）mRNA水平降低，但蜕皮类固醇滴度、体重或寿命没有改变。实验雌虫产卵前期延长，产卵量减少，孵化率和种群增长指数（PGI）降低。这种交配也导致雄性和雌性IRO发育受损，这通过免疫荧光染色得到证实。我们推断，SPF-L影响男性和他们的合作伙伴的生殖成功。
Selenoproteins serve in anti-oxidant and cellular redox functions in almost all organisms. A recent study characterized a selenoprotein F-like (SPF-L) in the brown plant hopper's (BPH), Nilaparvata lugens, male accessory glands (MAGs), raised the question of whether the SPF-L is associated with female fecundity. In this study, SPF-L mRNA was found to be enriched in the internal reproductive organ (IRO) of virgin males, also expressed relatively stably in virgin males and females, and dietary dsSPF-L-treatments led to reduced MAG protein and Arginine content. Knockdown of NlSPF-L in unmated males did not influence juvenile hormone (JH) III and ecdysteroid titers, however, dsSPF-L-treated mated males had increased JH III titer, and reduced ecdysteroid titer compared to controls. After mating with dsSPF-L-treated males, female partners had reduced fat body and ovary soluble proteins and JH III tier and vitellogenin (Vg) mRNA levels, but no alterations in ecdysteroid titer, body weight or longevity. The experimental females had prolonged pre-oviposition periods and they laid fewer eggs, which suffered reduced hatching rates and population growth index (PGI). Such mating also led to impaired IRO development in males and females, which was confirmed by immunofluorescence staining. We infer that SPF-L affects reproductive success of males and their partners.