Platelet dysfunction associated with experimental acute canine ehrlichiosis
Platelet dysfunction associated with experimental acute canine ehrlichiosis
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实验性急性犬埃立克体病相关的血小板功能障碍
DOI：
10.1136/vr.139.12.290
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发表时间：
1996
影响因子：
2.2
通讯作者：
H. Bark
中科院分区：
文献类型：
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作者：
S. Harrus;T. Waner;A. Eldor;E. Zwang;H. Bark
为了确定犬埃立克体病是否发生血小板功能障碍，采用间接法对6只实验感染犬埃立克体的犬的血清进行了血小板聚集反应研究，包括胶原/肾上腺素、凝血酶和二磷酸腺苷（ADP）。通过与血清阴性健康犬的富血小板血浆孵育，检测感染前和感染后4天和20天采集的血清样品。六只感染犬中有五只对至少一种所使用的激动剂产生反应，血小板聚集被显着抑制。在感染后20天采集的5只犬中的3只犬的血清中，记录到对胶原/肾上腺素的反应的预聚集滞后时间显著增加（P <0.05）。当与感染前的值相比时，检测到平均聚集前滞后时间显著增加了45%（P <0.05）。感染后4d和20d的一份血清样品对ADP的最大相对聚集反应显著降低（P <0.01），感染后20d的一份血清样品对ADP的最大相对聚集反应的斜率显著降低（P <0.05）。感染后第4天，其中2只犬的血清样品对凝血酶的最大相对聚集反应及其相对斜率显著降低（P <0.05）。结果表明，血小板功能障碍可能发生在犬埃立克体病的急性期，并可能是一个促成因素，往往在这种疾病中观察到出血。针对血小板糖蛋白的抗血小板抗体可能在抑制血小板聚集中起作用。
To determine whether platelet dysfunction occurs in canine ehrlichiosis, platelet aggregation studies in response to collagen/ epinephrine, thrombin and adenosine diphosphate (ADP) were carried out by the indirect method, using sera from six dogs experimentally infected with Ehrlichia canis. Samples of serum taken before infection and four and 20 days after infection were tested by incubation with platelet-rich plasma from a seronegative healthy dog. Platelet aggregation was significantly inhibited in five of six infected dogs in response to at least one of the agonists used. A significant increase in preaggregation lag time was recorded in response to collagen/ epinephrine in sera taken 20 days after infection from three of five dogs (P<0.05). When compared with the preinfection values, a significant increase of 45 per cent in the mean preaggregation lag time was detected (P<0.05). Maximal relative aggregation responses to ADP decreased significantly in one serum sample taken four days and one taken 20 days after infection (P<0.01) and there was a significantly lower relative slope for one serum sample 20 days after infection (P<0.05). Maximal relative aggregation responses to thrombin were significantly decreased together with their relative slopes in serum samples from two of four dogs four days after infection (P<0.05). The results suggest that platelet dysfunction may occur in the acute stage of canine ehrlichiosis, and may be a contributing factor to the tendency to bleed commonly observed in this disease. Antiplatelet antibodies directed against platelet glycoproteins may play a role in the inhibition of platelet aggregation.
R.G.Duggleby：“酶学方法”
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