Neuropeptide Y induces fasted pattern of duodenal motility via Y2 receptors in conscious fed rats
Neuropeptide Y induces fasted pattern of duodenal motility via Y2 receptors in conscious fed rats
复制标题
DOI：
10.1152/ajpgi.2000.278.1.g32
复制
发表时间：
2000-01-01
影响因子：
4.5
通讯作者：
Inui, A
中科院分区：
文献类型：
--
作者：
Fujimiya, M;Itoh, E;Inui, A
神经肽Y（neuropeptide Y，NPY）是一种由36个氨基酸组成的多肽，在脑内大量表达，参与摄食和内脏功能的调节。本研究的目的是调查是否NPY特异性调节十二指肠运动。测压法用于测量清醒、自由活动大鼠的十二指肠运动。大鼠十二指肠在禁食状态下表现出模仿移行运动复合体的阶段性收缩，在摄入食物后被不规则收缩所取代。神经肽Y强有力地影响脑室内（icv）给药后的收缩活动，将进食（餐后）模式转变为以禁食（消化间期）模式为特征的阶段性收缩。这种作用是通过与大鼠Y-2和Y-4受体药理学特征相似的受体介导的，尽管Y-1和Y-5激动剂都对运动性没有任何影响，尽管具有强有力的摄食刺激作用。免疫中和与抗NPY抗血清给予冰冷废除禁食模式，并诱导进食样运动活动。一个冰冷的剂量的肽YY产生了不同的影响，从NPY，增加运动活动的喂养和禁食模式。这些结果表明，禁食和进食的运动活动的调节过程，神经肽Y诱导禁食活动通过Y-2，并可能Y-4，受体，这可能是一个综合的机制与进食行为的发病。
Neuropeptide Y (NPY), a 36-amino acid peptide abundantly expressed in the brain, has been implicated in the regulation of feeding and visceral functions. The present study was designed to investigate whether or not NPY specifically regulates duodenal motility. The manometric method was used to measure duodenal motility in conscious, freely moving rats. The rat duodenum showed phasic contractions mimicking the migrating motor complex in the fasted state that were replaced by irregular contractions after the ingestion of food. NPY powerfully affected the contractile activity after intracerebroventricular (icv) administration, changing fed (postprandial) patter ns into phasic contractions characterized as fasted (interdigestive) patterns. This effect was mediated via receptors with pharmacological profiles similar to rat Y-2 and Y-4 receptors, although neither Y-1 nor Y-5 agonists had any effects on motility despite potent feeding-stimulatory effects. Immunoneutralization with anti-NPY antiserum administered icy abolished fasted patterns and induced fed-like motor activities. An icy dose of peptide YY produced a different effect from NPY, with increase in the motor activities of both fed and fasted patterns. These results indicate that fasted and fed motor activities are regulated processes and that NPY induces fasted activity through Y-2, and possibly Y-4, receptors, which may represent an integrated mechanism linked to the onset of feeding behavior.