In vitro cytoprotective effect of infant milk formula fortified with human rotavirus-specific hyperimmune yolk immunoglobulins (IgY)
In vitro cytoprotective effect of infant milk formula fortified with human rotavirus-specific hyperimmune yolk immunoglobulins (IgY)
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DOI：
10.1007/s10068-013-0269-4
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发表时间：
2013-10
影响因子：
2.9
通讯作者：
Shofiqur Rahman;Kouji Umeda;F. Icatlo;K. Lee;S. H. Kim;Hyuk-Joon Choi;Bok-Kyung Han;Young-Seo Park;Nguyen Van Sa
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文献类型：
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作者：
Shofiqur Rahman;Kouji Umeda;F. Icatlo;K. Lee;S. H. Kim;Hyuk-Joon Choi;Bok-Kyung Han;Young-Seo Park;Nguyen Van Sa
对添加抗人轮状病毒（HRV）高免疫球蛋白Y（IgY）的婴儿配方奶粉进行体外抗HRV临床菌株ATCC VR 2273和ATCC VR 2274的评价。制备了特异性抗人轮状病毒抗体（Rotamix IgY）。评价了Rotamix IgY单独使用以及与婴儿配方奶粉预混溶液在中和MA 104细胞中轮状病毒感染性方面的有效性。使用50%荧光焦点（FF）抑制试验，试验婴儿配方奶粉对不同的人轮状病毒株具有强烈的交叉反应，滴度为80-320。Rotamix IgY单独和与婴儿配方奶粉的预混溶液均显示出对主要轮状病毒全球血清型G1、G2、G3和G4单独以及与其他人和动物毒株的多血清型交叉中和活性。与对照IgY补充的婴儿配方奶粉相比，轮状病毒感染引起的细胞-轮状病毒粘附和细胞损伤以剂量依赖性方式被显著抑制。
Infant formula supplemented with hyperimmune immunoglobulin Y (IgY) against human rotavirus (HRV) was evaluatedin vitroagainst HRV reassortant clinical strains ATCC VR 2273 and ATCC VR 2274. Specific antihuman rotavirus antibody powder (Rotamix IgY) was prepared. The effectiveness of Rotamix IgY alone and as a pre-mixed solution with infant formula was evaluated for neutralizing rotavirus infectivity in MA104 cells. The test infant formula cross-reacted strongly against different human rotavirus strains with titers of 80-320 using a 50% fluorescent focus (FF) inhibition test. Both rotamix IgY alone and in a pre-mixed solution with infant formula showed multi-serotypic cross neutralization activities against the major rotavirus global serotypes G1, G2, G3, and G4 alone and with other human and animal-strainsin vitro. Cell-rotavirus adhesion and cell damage arising from rotavirus infection were significantly inhibited in a dose dependent manner, compared to control IgY supplemented infant formula.