Forward signaling by EphB1/EphB2 interacting with ephrin-B ligands at the optic chiasm is required to form the ipsilateral projection.
Forward signaling by EphB1/EphB2 interacting with ephrin-B ligands at the optic chiasm is required to form the ipsilateral projection.
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DOI：
10.1111/j.1460-9568.2011.07845.x
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发表时间：
2011-11
期刊：
影响因子：
--
通讯作者：
Henkemeyer M
中科院分区：
文献类型：
--
作者：
Chenaux G;Henkemeyer M
EphB受体酪氨酸激酶通过轴突-细胞接触后与肝配蛋白-B蛋白的相互作用指导轴突寻路。由于EphB：肝配蛋白-B结合导致双向信号，因此不能使用传统的蛋白质无效等位基因来分配信号传导到表达Eh的细胞（正向信号传导）或表达肝配蛋白的细胞（反向信号传导）中的贡献。为了确定EphB 1在轴突寻路过程中是否仅作为受体发挥作用，创建了一种新的敲入突变小鼠EphB 1 T-lacZ，其从内源基因座表达细胞内截短的EphB 1-β-gal融合蛋白。与EphB 1 −/−蛋白缺失动物一样，EphB 1 T-lacZ/T-lacZ纯合子不能形成视网膜神经节细胞轴突的同侧投射亚群。这表明通过EphB 1的细胞外结构域的反向信号传导对于视交叉处视网膜轴突的正确轴突寻路不是必需的。对其他EphB和肝配蛋白-B突变小鼠的进一步分析表明，EphB 1是肝配蛋白-B2的优选受体，并且在较小程度上，肝配蛋白-B1在视交叉处介导轴突引导，尽管EphB 2在相同的同侧投射视网膜轴突中共表达。
EphB receptor tyrosine kinases direct axonal pathfinding through interactions with ephrin-B proteins following axon-cell contact. Since EphB:ephrin-B binding leads to bidirectional signals, the contributions of signaling into the Eph-expressing cell (forward signaling) or the ephrin-expressing cell (reverse signaling) cannot be assigned using traditional protein-null alleles. To determine if EphB1 is functioning solely as a receptor during axon pathfinding, a new knock-in mutant mouse was created, EphB1T-lacZ, that expresses an intracellular-truncated EphB1-β-gal fusion protein from the endogenous locus. As in the EphB1−/− protein-null animals, the EphB1T-lacZ/T-lacZ homozygotes fail to form the ipsilateral projecting subpopulation of retinal ganglion cell axons. This indicates that reverse signaling through the extracellular domain of EphB1 is not required for proper axon pathfinding of retinal axons at the optic chiasm. Further analysis of other EphB and ephrin-B mutant mice shows that EphB1 is the preferred receptor of ephrin-B2 and, to a lesser degree, ephrin-B1 in mediating axon guidance at the optic chiasm despite the coexpression of EphB2 in the same ipsilaterally projecting retinal axons.
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影响因子：
64.8
作者：
Himanen, JP;Rajashankar, KR;Nikolov, DB
通讯作者：
Nikolov, DB
多个EPHB受体酪氨酸激酶在海马中塑造树突状刺。
影响因子：
7.8
作者：
Henkemeyer, Mark;Itkis, Olga S;Ngo, Michelle;Hickmott, Peter W;Ethell, Iryna M
通讯作者：
Ethell, Iryna M
影响因子：
64.5
作者：
Henkemeyer, M;Orioli, D;Klein, R
通讯作者：
Klein, R
影响因子：
30.5
作者：
通讯作者：
--
DOI：
10.1098/rspb.1985.0021
发表时间：
1985-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DRAGER, UC
通讯作者：
DRAGER, UC