Isolation of Splenic Dendritic Cells Using Fluorescence-activated Cell Sorting.
Isolation of Splenic Dendritic Cells Using Fluorescence-activated Cell Sorting.
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DOI：
10.21769/bioprotoc.1415
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发表时间：
2015-03
期刊：
影响因子：
0.8
通讯作者：
S. Tavernier;F. Osorio;S. Janssens;B. Lambrecht
中科院分区：
文献类型：
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作者：
S. Tavernier;F. Osorio;S. Janssens;B. Lambrecht
脾脏是一个血管丰富的器官，由先天性和适应性免疫细胞组成的复杂组织网络。这使得脾脏的特殊功能，如抗细菌和抗真菌免疫和铁代谢等（Mebius和Kraal，2005）。不同的树突状细胞（DC）亚群存在于脾脏中，并且可以通过独特的表面标志物的表达来定义。这些DC亚群被识别为在免疫系统中执行非冗余功能（Merad et al.，2013年）的报告。在我们最近的研究中，我们发现肌醇需要酶（IRE）-1在脾CD 8a + DC中特异性活化。此外，X-box结合蛋白（XBP）-1（由IRE-1调节的转录因子）的缺失导致脾CD 8a + DC对死细胞相关抗原的交叉呈递缺陷（Osorio等人，2014）。该方案允许离体分离用于实验用途的特定DC亚群。
The spleen is a vastly vasculated organ and consists of a complex organized network of innate and adaptive immune cells. This permits the specialized functions of the spleen such as antibacterial and antifungal immunity and iron metabolism among others (Mebius and Kraal, 2005). Different dendritic cell (DC) subsets reside in the spleen and can be defined by the expression of unique surface markers. These DC subsets are recognized to perform non-redundant functions in the immune system (Merad et al., 2013). In our recent study, we found that Inositol Requiring Enzyme (IRE)-1 is specifically activated in splenic CD8a+ DCs. Furthermore, loss of X-box binding protein (XBP)-1 - the transcription factor regulated by IRE-1 - resulted in defective cross-presentation of dead cell associated antigens by splenic CD8a+ DCs (Osorio et al., 2014). This protocol allows the isolation of specific DC subsets for experimental use ex-vivo.