Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis:: identification of its responsible molecules in translation products of rgpA, kgp, and hagA genes
Roles of Arg- and Lys-gingipains in coaggregation of Porphyromonas gingivalis:: identification of its responsible molecules in translation products of rgpA, kgp, and hagA genes
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DOI：
10.1515/bc.2004.135
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发表时间：
2004-11-01
影响因子：
3.7
通讯作者：
Yamamoto, K
中科院分区：
文献类型：
--
作者：
Abe, N;Baba, A;Yamamoto, K
牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种口腔厌氧菌，其分泌的精氨酸（Rgp）和赖氨酸（Lys-gingipains，Kgp）是两种半胱氨酸蛋白酶，是引起牙周炎的主要致病因子。这种细菌与其他口腔细菌的共聚集是感染过程中的初始和关键步骤，但负责这一过程的因素和机制仍然难以捉摸。在这里，我们表明，最初的翻译产物的rgpA，Kgp和血凝素hagA基因是负责牙龈卟啉单胞菌的共聚集和Rgp和Kgp的蛋白水解活性是必不可少的，在这个过程中。rgpA rgpB kgp和rgpA kgp hagA缺陷的三重突变体与粘性放线菌没有共聚集活性，而kgp无效和rgpA rgpB缺陷的双突变体显着保留了这种活性。一致的是，Rgp和Kgp特异性抑制剂的联合作用强烈抑制了细菌的共聚集活性，尽管单独使用Rgp或Kgp特异性抑制剂显著保留了这种活性。我们还表明，47-和43-kDa的蛋白质产生的翻译产物的rgpA，kgp和hagA基因的蛋白水解活性的Rgp和Kgp是负责的牙龈卟啉单胞菌的共聚集。
Arg- (Rgp) and Lys-gingipains (Kgp) are two individual cysteine proteinases produced by Porphyromonas gingivalis, an oral anaerobic bacterium, and are implicated as major virulence factors in a wide range of pathologies of adult periodontitis. Coaggregation of this bacterium with other oral bacteria is an initial and critical step in infectious processes, yet the factors and mechanisms responsible for this process remain elusive. Here we show that the initial translation products of the rgpA, kgp and hemagglutinin hagA genes are responsible for coaggregation of P gingivalis and that the proteolytic activity of Rgp and Kgp is indispensable in this process. The rgpA rgpB kgp- and rgpA kgp hagA-deficient triple mutants exhibited no coaggregation activity with Actinomyces viscosus, whereas the kgp-null and rgpA rgpB-deficient double mutants significantly retained this activity. Consistently, the combined action of Rgp- and Kgp-specific inhibitors strongly inhibited the coaggregation activity of the bacterium, although single use of Rgp- or Kgp-specific inhibitor significantly retained this activity. We also demonstrate that the 47- and 43-kDa proteins produced from the translation products of the rgpA, kgp, and hagA genes by proteolytic activity of both Rgp and Kgp are responsible for the coaggregation of P. gingivalis.