Mast cell adhesion to fibronectin.
Mast cell adhesion to fibronectin.
复制标题
肥大细胞粘附纤连蛋白。
作者：
J. Dastych;J. J. Costa;H. Thompson;D. Metcalfe
MCP-5鼠肥大细胞系，以及原代骨髓来源的培养肥大细胞（BMCMC），被证明结合到纤连蛋白，细胞外基质的普遍存在的粘附蛋白。BMCMC需要激活佛波醇肉豆蔻酸酯醋酸酯（PMA）坚持纤连蛋白，而MCP-5显示自发粘附。MCP-5和BMCMC两者的结合是剂量依赖性的，在纤连蛋白浓度为20微克/ml时具有最大粘附。含有RGDS细胞附着位点的纤连蛋白的120，000分子量（MW）蛋白水解片段能够替代天然纤连蛋白分子促进肥大细胞附着。此外，RGDS肽单独可抑制肥大细胞与纤连蛋白的粘附。这些数据表明，除了先前描述的肥大细胞层粘连蛋白的相互作用，肥大细胞也坚持纤连蛋白，从而提供了进一步了解其组织定位和可能的作用过程中，如伤口愈合和纤维化。
The MCP-5 murine mast cell line, as well as primary bone marrow-derived cultured mast cells (BMCMC), are demonstrated to bind to fibronectin, a ubiquitous adhesion protein of the extracellular matrix. BMCMC required activation by phorbol myristate acetate (PMA) to adhere to fibronectin, whereas MCP-5 displayed spontaneous adherence. The binding of both MCP-5 and BMCMC was dose dependent, with maximal adhesion at a fibronectin concentration of 20 micrograms/ml. The 120,000 molecular weight (MW) proteolytic fragment of fibronectin containing the RGDS cell attachment site was able to substitute for the native fibronectin molecule in promoting mast cell attachment. Mast cell adhesion to fibronectin, in addition, could be inhibited by the RGDS peptide alone. These data suggest that, in addition to the previously described mast cell-laminin interactions, mast cells also adhere to fibronectin, thus providing further insight into their tissue localization and possible roles in processes such as wound healing and fibrosis.