Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis.
Metabolic reconstruction of sulfur assimilation in the extremophile Acidithiobacillus ferrooxidans based on genome analysis.
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DOI：
10.1186/1471-2164-4-51
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发表时间：
2003-12-15
期刊：
影响因子：
4.4
通讯作者：
Holmes D
中科院分区：
文献类型：
--
作者：
Valdés J;Veloso F;Jedlicki E;Holmes D
酸硫胆杆菌铁氧化物是一种伽马杆菌，它居住在PH2上，并通过硫和铁的氧化而获得能量。它用于生物启动工业中的金属回收，是酸性矿山排水的致病药物之一。研究其遗传学和生理学的有效工具并非广泛使用，尽管大力努力，但对其异常生理学的理解仍处于基本水平。可以从两个公共资源获得几乎完整的基因组序列，我们利用了这些信息来重建其硫代谢的方面。
检测到了从环境吸收硫酸盐吸收的两种候选机制，但两者都属于膜转运蛋白的大型寄生虫家族，其鉴定仍然是暂时的。鉴定出可能与硫酸盐同化为半胱氨酸并形成Fe-S中心的前瞻性基因，途径和调节机制。还发现了基因和调节网络，这些网络可能会将硫同化与氮固定，氢利用和硫酸还原联系起来。鉴定了潜在的途径，用于细胞外代谢产物的硫酸化，这些硫酸盐可能与黄铁矿，硫和其他固体底物有关。
对铁曲霉的基因组序列的生物信息学分析揭示了候选基因，代谢过程和控制机制可能与硫代谢方面有关。代谢建模为理解这种极端细胞的异常生理提供了重要的初步步骤，特别是考虑到其遗传操作和生化分析涉及的严重困难。
Acidithiobacillus ferrooxidans is a gamma-proteobacterium that lives at pH2 and obtains energy by the oxidation of sulfur and iron. It is used in the biomining industry for the recovery of metals and is one of the causative agents of acid mine drainage. Effective tools for the study of its genetics and physiology are not in widespread use and, despite considerable effort, an understanding of its unusual physiology remains at a rudimentary level. Nearly complete genome sequences of A. ferrooxidans are available from two public sources and we have exploited this information to reconstruct aspects of its sulfur metabolism. Two candidate mechanisms for sulfate uptake from the environment were detected but both belong to large paralogous families of membrane transporters and their identification remains tentative. Prospective genes, pathways and regulatory mechanisms were identified that are likely to be involved in the assimilation of sulfate into cysteine and in the formation of Fe-S centers. Genes and regulatory networks were also uncovered that may link sulfur assimilation with nitrogen fixation, hydrogen utilization and sulfur reduction. Potential pathways were identified for sulfation of extracellular metabolites that may possibly be involved in cellular attachment to pyrite, sulfur and other solid substrates. A bioinformatic analysis of the genome sequence of A. ferrooxidans has revealed candidate genes, metabolic process and control mechanisms potentially involved in aspects of sulfur metabolism. Metabolic modeling provides an important preliminary step in understanding the unusual physiology of this extremophile especially given the severe difficulties involved in its genetic manipulation and biochemical analysis.
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影响因子：
3.2
作者：
HRYNIEWICZ, MM;KREDICH, NM
通讯作者：
KREDICH, NM
影响因子：
3.2
作者：
Keating, DH;Willits, MG;Long, SR
通讯作者：
Long, SR
影响因子：
4.7
作者：
Barreto, M;Quatrini, R;Holmes, DS
通讯作者：
Holmes, DS
DOI：
10.1111/j.1432-1033.1995.347_1.x
发表时间：
1995-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
BERENDT, U;HAVERKAMP, T;SCHWENN, JD
通讯作者：
SCHWENN, JD
影响因子：
5.6
作者：
Jones, DT
通讯作者：
Jones, DT