Transcytosis of infectious human immunodeficiency virus across a tight human epithelial cell line barrier
Transcytosis of infectious human immunodeficiency virus across a tight human epithelial cell line barrier
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DOI：
10.1038/nm0197-42
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发表时间：
1997-01-01
期刊：
影响因子：
82.9
通讯作者：
Bomsel, M
中科院分区：
文献类型：
--
作者：
Bomsel, M
各种上皮细胞系与HIV慢性感染的单核细胞系接触，导致HIV病毒粒子向上皮大量快速出芽，然后内化到上皮内体样结构中。研究表明，早在根尖接触30分钟后，HIV感染者外周血白细胞产生的原代病毒分离物就可以通过胞吞作用越过上皮细胞系屏障。典型的上皮细胞囊泡通路。作为感染扩散的下一步，经胞切的HIV颗粒可以有效地感染位于上皮屏障基底外侧的单个核细胞。这些观察结果提示了HIV通过完整上皮屏障传播的另一种快速有效的机制。
Contact between various epithelial cell lines and HIV chronically infected mononuclear cell lines results in a massive and rapid budding of HIV virions toward the epithelium followed by their internalization into epithelial endosome-like structures, Here it is shown that as early as 30 minutes after apical contact, primary virus isolates generated from primary peripheral blood leukocytes from HIV-infected patients can cross an epithelial cell line barrier using transcytosis, the characteristic epithelial transcellular vesicular pathway. As the next step in the spread of infection, transcytosed HIV particles can productively infect mononuclear cells located at the basolateral side of the epithelial barrier. These observations suggest an alternative, rapid and efficient mechanism for transmission of HIV across an intact epithelial barrier.