High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging
High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging
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DOI：
10.1364/ol.28.002064
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发表时间：
2003-11-01
期刊：
影响因子：
3.6
通讯作者：
Fujimoto, JG
中科院分区：
文献类型：
--
作者：
Aguirre, AD;Hsiung, P;Fujimoto, JG
利用高速宽带反射栅位相调制器和飞秒Ti：Al_2O_3激光器实现了光学相干显微镜(OCM)。这种新颖的系统设计允许在新的工作模式下进行高分辨率的OCM成像，其中使用了短相干门来放宽对高数值孔径共焦轴向剖切的要求。在活体细胞成像中展示了非洲爪哇蝌蚪和人类皮肤的3微米相干门和30微米共焦门。用较小的数值孔径实现细胞成像的能力应该有助于开发用于活体成像应用的小型化探针。(C)2003年美国光学学会。
Optical coherence microscopy (OCM) is demonstrated with a high-speed, broadband, reflective-grating phase modulator and a femtosecond Ti:Al2O3 laser. The novel system design permits high-resolution OCM imaging in a new operating regime in which a short coherence gate is used to relax the requirement for high-numerical-aperture confocal axial sectioning. In vivo cellular imaging is demonstrated in the Xenopus laevis tadpole and in human skin with a 3-mum coherence gate and a 30-mum confocal gate. The ability to achieve cellular imaging with a lower numerical aperture should facilitate the development of miniaturized probes for in vivo imaging applications. (C) 2003 Optical Society of America.