Expression of ATP synthase CF1 alpha subunit gene (CTL-spn) as screened by the cDNA-SRAP approach is correlated with spininess in Carthamus tinctorius L.
Expression of ATP synthase CF1 alpha subunit gene (CTL-spn) as screened by the cDNA-SRAP approach is correlated with spininess in Carthamus tinctorius L.
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通过 cDNA-SRAP 方法筛选的 ATP 合成酶 CF1 α 亚基基因 (CTL-spn) 的表达与红花的刺状相关。
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发表时间：
2015-08
期刊：
影响因子：
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通讯作者：
Meili Guo
中科院分区：
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作者：
D;an Guo;Qinghua Guo;Yue Gao;Meili Guo
红花是一种中药，用于活血化瘀，疏通经络。当人工收割时，其苞片上的刺被认为是一个障碍。在这项研究中，利用dna - srap系统地研究了哪些基因与棘相关。60对引物组合分别用于有刺和无刺两个cDNA库。共鉴定出6个转录源片段，其中重组率较低的片段测序成功，命名为GPY-1和GPY-2。利用RACE方法克隆GPY-2全长cDNA，命名为CTL-spn。CTL-spn全长1 679 bp， ORF全长1 524 bp，编码一个508个氨基酸的蛋白。推断的CTL-spn基因的氨基酸序列与其他已知的ATP合成酶CF1 α亚基具有高度同源性（97%）。半定量RT-PCR分析显示，GPY-1和GPY-2 mRNA仅在棘系中积累。考虑到ATP合成酶CF1 α亚基在植物中的重要作用，它可能直接参与多刺红花的多刺形成过程，增强多刺红花的抗性反应。研究结果为红花无刺品种的选育提供了重要的参考。
The safflower floret is a traditional Chinese medicine used to promote blood circulation and remove obstruction in the channels. The spines on its bracts are considered a handicap when manual harvest is involved. In this study, cDNA-SRAP was used to systematically investigate which genes are associated with the spines. Sixty pairs of possible primer combinations were used on two cDNA pools representing spininess and spinelessness. Six transcript-derived fragments were identified, of which two with low recombination were sequenced successfully and named as GPY-1 and GPY-2. By using the RACE method, the full-length cDNA of GPY-2 is cloned and named as CTL-spn. The full-length cDNA of CTL-spn was 1 679 bp long with a 1 524 bp ORF encoding a 508 aminoacid protein. The deduced amino acid sequence of the CTL-spn gene shared a high homology (97%) with other known ATP synthase CF1 alpha subunits. Semiquantitative RT-PCR analysis revealed that the mRNA of GPY-1 and GPY-2 accumulated in only spiny lines. Considering the important role of ATP synthase CF1 alpha subunit in plants, it may directly take part in the formation process of spininess and enhancing resistance reaction of spiny safflower. Also, our results provide the important insights for breeding spineless cultivars of safflower.