Visualization of lymphatic vessels by Prox1-promoter directed GFP reporter in a bacterial artificial chromosome-based transgenic mouse
Visualization of lymphatic vessels by Prox1-promoter directed GFP reporter in a bacterial artificial chromosome-based transgenic mouse
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DOI：
10.1182/blood-2010-07-298562
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发表时间：
2011-01-06
期刊：
影响因子：
20.3
通讯作者：
Hong, Young-Kwon
中科院分区：
文献类型：
--
作者：
Choi, Inho;Chung, Hee Kyoung;Hong, Young-Kwon
虽然血管特异性荧光转基因小鼠已经成为研究血管发生和血管生成的良好工具，但迄今为止尚未建立血管特异性荧光小鼠模型。在这里，我们报告了一个转基因动物模型，表达的绿色荧光蛋白的启动子下的Prox 1，一个主控制基因在淋巴发育。使用大约200 kb长的细菌人工染色体携带整个Prox 1基因，这种Prox 1-绿色荧光蛋白小鼠被发现忠实地概括了淋巴管内皮细胞和其他Prox 1表达器官中Prox 1基因的表达模式，使我们能够方便地可视化整个发育过程中淋巴管和网络的详细结构和形态。我们的数据表明，这种新的转基因小鼠可以非常有用的检测，成像和分离淋巴管和监测伤口相关的淋巴管生成。总之，这种Prox 1-绿色荧光蛋白转基因小鼠将成为淋巴研究的一个很好的工具。（血。2011;117（1）：362-365）
Although the blood vessel-specific fluorescent transgenic mouse has been an excellent tool to study vasculogenesis and angiogenesis, a lymphatic-specific fluorescent mouse model has not been established to date. Here we report a transgenic animal model that expresses the green fluorescent protein under the promoter of Prox1, a master control gene in lymphatic development. Generated using an approximately 200-kb-long bacterial artificial chromosome harboring the entire Prox1 gene, this Prox1-green fluorescent protein mouse was found to faithfully recapitulate the expression pattern of the Prox1 gene in lymphatic endothelial cells and other Prox1-expressing organs, and enabled us to conveniently visualize detailed structure and morphology of lymphatic vessels and networks throughout development. Our data demonstrate that this novel transgenic mouse can be extremely useful for detection, imaging, and isolation of lymphatic vessels and monitoring wound-associated lymphangiogenesis. Together, this Prox1-green fluorescent protein transgenic mouse will be a great tool for the lymphatic research. (Blood. 2011;117(1):362-365)