Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening
Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening
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DOI：
10.1016/s0092-8674(00)81908-x
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发表时间：
1997-03-07
期刊：
影响因子：
64.5
通讯作者：
Langmore, JP
中科院分区：
文献类型：
--
作者：
Makarov, VL;Hirose, Y;Langmore, JP
The chromosomes of lower eukaryotes have short telomeric 3' extensions. Using a primer-extension/nick-translation technique and nondenaturing hybridization, we find long 3' G-rich tails at human chromosome ends in mortal primary fibroblasts, umbilical vein endothelial cells, and leukocytes, as well as in immortalized fibroblasts. For all cells tested, >80% of the telomeres have long G-rich overhangs, averaging 130-210 bases in length, in disagreement with the conventional model for incomplete lagging-strand replication, which predicts overhangs on 50% of the chromosome ends. The observed G tails must exist during most of the cell cycle and probably result from degradation of both chromosome ends. The average lengths of the G tails are quantitatively consistent with the observed rates of human chromosome shortening.
