P-ELEMENT DISTRIBUTION IN EURASIAN POPULATIONS OF DROSOPHILA-MELANOGASTER - A GENETIC AND MOLECULAR ANALYSIS
P-ELEMENT DISTRIBUTION IN EURASIAN POPULATIONS OF DROSOPHILA-MELANOGASTER - A GENETIC AND MOLECULAR ANALYSIS
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DOI：
10.1073/pnas.82.16.5418
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
TCHEN, P
中科院分区：
文献类型：
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作者：
ANXOLABEHERE, D;NOUAUD, D;TCHEN, P
对欧亚黑腹果蝇群体的杂种发育不全的 P-M 系统进行了遗传和分子研究。在从法国到中亚采样的 27 个菌株中，西欧和东部地区之间存在明显的梯度，其中大多数现代菌株为 Q 型，而东部地区则以 M 细胞型菌株为主。使用克隆的 2.9 碱基 P 元件的 2 个互补探针对单个果蝇进行分子分析。结果提供了P元素频率从西到东逐渐降低的证据，但在所有分析的野生M细胞型群体中都确定了P同源序列的存在。当用高度敏感的参考系进行测试时，在大多数 M 细胞型群体中发现了一些具有 GD 不育潜力的活性 P 元件。结合元素结构以及P-M进化理论讨论了欧亚大陆多态性P家族分布的逐渐变化，并将其解释为这些元素目前对欧亚种群的入侵。
Genetic and molecular investigations were carried out with Eurasian D. melanogaster populations on the P-M system of hybrid dysgenesis. In 27 strains sampled from France to Middle Asia, a clear gradient exists between Western Europe, in which most modern strains are of the Q type, and eastern areas, in which M-cytotype strains predominate. Molecular analysis on individual flies was performed with 2 complementary probes of the cloned 2.9-kilobase P element. The results provide evidence for a gradually decreasing frequency of P elements from west to east, but the presence of P-homologous sequences was ascertained in all of the wild M-cytotype populations analyzed. Some active P elements with GD sterility potential were revealed in the majority of M-cytotype populations when tested with a highly sensitive reference line. The gradual change in distribution of the polymorphic P family in Eurasia is discussed in relation to the structure of the elements together with the theories of P-M evolution and is interpreted as the present invasion of Eurasian populations by these elements.