Ly 9, an alloantigenic marker of lymphocyte differentiation.
Ly 9, an alloantigenic marker of lymphocyte differentiation.
复制标题
Ly 9，淋巴细胞分化的同种异体抗原标记。
作者：
B. Mathieson;S. Sharrow;K. Bottomly;B. Fowlkes
一种新的淋巴细胞表面同种异体抗原，暂时命名为Ly 9，通过流式显微荧光测定法（FMF）检测，作为抗Lyt免疫血清中的额外特异性。Ly 9.2是一种等位基因特异性，通常被检测为胸腺细胞与抗Lyt 3.1血清的独特免疫荧光谱。替代抗原特异性Ly 9.1可在针对Lyt 2.1和Lyt 3.2制备的抗血清中常规检测到。这种淋巴细胞同种异体抗原具有独特的菌株和细胞/组织分布。通过FMF对杂合子细胞的Ly 9免疫荧光的定量测量显示Ly 9抗原表达是共显性的，并且相对于亲本纯合子细胞水平降低。Ly 9在所有胸腺细胞、外周淋巴细胞和骨髓细胞亚群上表达。该抗原在红细胞、表皮细胞、精子或睾丸、脑、肾、肝或肺的悬浮液中不表达至任何显著程度。FMF分析和吸收分型揭示了与脾或淋巴结细胞相比，胸腺细胞上的抗原表达水平在数量上较低。细胞毒性消除实验证实Ly 9在至少2种不同的T细胞功能亚群和B细胞上表达。
A new lymphocyte cell surface alloantigen, provisionally designated Ly 9, is detected by flow microfluorometry(FMF), as an extra specificity in sera from anti-Lyt immunizations. Ly 9.2, one of the allelic specificities, commonly is detected as a unique immunofluorescence profile of thymocytes with anti-Lyt 3.1 sera. The alternative antigenic specificity, Ly 9.1, can be detected routinely in antisera prepared against Lyt 2.1 and Lyt 3.2. This lymphocyte alloantigen has both a unique strain and cell/tissue distribution. Quantitative measurement of Ly 9 immunofluorescence by FMF on heterozygote cells shows that Ly 9 antigen expression is co-dominant and reduced in levels relative to parental homozygote cells. Ly 9 is expressed on all thymocytes, peripheral lymphocytes, and on a subpopulation of cells in the bone marrow. This antigen is not expressed to any significant degree on erythrocytes, epidermal cells, sperm, or in suspensions of testis, brain, kidney, liver, or lung. FMF analysis and absorption typing reveals a quantitatively lower level of antigen expression on thymocytes compared with spleen or lymph node cells. Cytotoxic elimination experiments confirm that Ly 9 is expressed on at least 2 different T cell functional subsets and B cells.