Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation.
Saccharomyces cerevisiae SMT4 encodes an evolutionarily conserved protease with a role in chromosome condensation regulation.
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发表时间：
2001-05
期刊：
影响因子：
3.3
通讯作者：
Alexander V. Strunnikov;L. Aravind;E. V. Koonin
中科院分区：
文献类型：
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作者：
Alexander V. Strunnikov;L. Aravind;E. V. Koonin
在寻找影响酿酒酵母中凝聚素复合物靶向染色质的调控基因时，我们确定了腺病毒蛋白酶家族的一个成员，SMT 4。SMT 4过表达抑制smc 2 -6的温度敏感性条件致死表型，但不抑制smc 2 -8或smc 4 -1。SMT 4的破坏等位基因具有突出的染色体表型：Smc 4p-GFP对rDNA染色质的靶向受损。预测的蛋白酶活性位点半胱氨酸和组氨酸残基的Smt 4p的定点突变废除SMT 4在体内的功能。先前未表征的SIZ 1（SAP和Miz）基因，其编码含有预测的DNA结合SAP模块和Miz指的蛋白质，被鉴定为与SMT 4破坏相关的生长缺陷的旁路抑制因子。SIZ 1基因破坏与SIZ 2缺失是合成致死的。我们认为SMT 4、SIZ 1和SIZ 2参与了一种新的染色体维持途径。
In a search for regulatory genes affecting the targeting of the condensin complex to chromatin in Saccharomyces cerevisiae, we identified a member of the adenovirus protease family, SMT4. SMT4 overexpression suppresses the temperature-sensitive conditional lethal phenotype of smc2-6, but not smc2-8 or smc4-1. A disruption allele of SMT4 has a prominent chromosome phenotype: impaired targeting of Smc4p-GFP to rDNA chromatin. Site-specific mutagenesis of the predicted protease active site cysteine and histidine residues of Smt4p abolishes the SMT4 function in vivo. The previously uncharacterized SIZ1 (SAP and Miz) gene, which encodes a protein containing a predicted DNA-binding SAP module and a Miz finger, is identified as a bypass suppressor of the growth defect associated with the SMT4 disruption. The SIZ1 gene disruption is synthetically lethal with the SIZ2 deletion. We propose that SMT4, SIZ1, and SIZ2 are involved in a novel pathway of chromosome maintenance.