Different apolipoproteins impact nanolipoprotein particle formation
Different apolipoproteins impact nanolipoprotein particle formation
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DOI：
10.1021/ja074753y
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发表时间：
2007-11-21
影响因子：
15
通讯作者：
Hoeprich, Paul D.
中科院分区：
文献类型：
--
作者：
Chromy, Brett A.;Arroyo, Erin;Hoeprich, Paul D.
纯化的载脂蛋白和磷脂的自发相互作用导致形成纳米尺寸的脂蛋白颗粒；我们将这些组装称为纳米载脂蛋白颗粒或NLP。这些双层结构可以作为生物膜的合适的模拟物，并且在水环境中完全溶解。本研究以家蚕蛾的载脂蛋白ApoA-1、Delta-apoA-I片段、apoE4片段和apolipophorin III(apoLp-III)为原料，分别与二肉豆蔻基磷脂酰胆碱(DMPC)和四种不同的载脂蛋白(apoA-1、Delta-apoA-I片段、apoE4片段和apoLp-III)结合，制备了NLP。这些颗粒以盘状为主，直径在10到20 nm之间，高度在4.5到5 nm之间，并且可以生产出产量在40%到60%之间的颗粒。特定的脂蛋白、脂脂比和组装参数决定了纳米脂蛋白颗粒的大小和均一性，表明apoA-I NLP制剂比较大的apoE422K和apolp-III NLP制剂小。
Spontaneous interaction of purified apolipoproteins and phospholipids results in formation of lipoprotein particles with nanometer-sized dimensions; we refer to these assemblies as nanolipoprotein particles or NLPs. These bilayer constructs can serve as suitable mimetics of biological membranes and are fully soluble in aqueous environments. We made NLPs from dimyristoylphospatidylcholine (DMPC) in combination with each of four different apolipoproteins: apoA-1, Delta-apoA-I fragment, apoE4 fragment, and apolipophorin III (apoLp-III) from the silk moth B. mori. Predominately discoidal in shape, these particles have diameters between 10 and 20 nm, share uniform heights between 4.5 and 5 nm, and can be produced in yields ranging between 40 and 60%. The particular lipoprotein, the lipid to lipoprotein ratio, and the assembly parameters determine the size and homogeneity of nanolipoprotein particles and indicate that apoA-I NLP preparations are smaller than the larger apoE422K and apolp-III NLP preparations.