Assembling complex genotypes to resist Fusarium in wheat (Triticum aestivum L.)
Assembling complex genotypes to resist Fusarium in wheat (Triticum aestivum L.)
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DOI：
10.1007/s00122-005-0094-0
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发表时间：
2005-11-01
影响因子：
5.4
通讯作者：
Fedak, G
中科院分区：
文献类型：
--
作者：
Somers, DJ;Thomas, J;Fedak, G
小麦赤霉病是全球小麦生产的主要障碍。小麦中FHB抗性的遗传学正在变得清晰，并且对来自不同来源（例如Sumai3、Wuhan、Nyubai和Frontana）的FHB抗性QTL的基因组位置有了很好的了解。通过大规模分子育种实验组装复杂基因型所需的所有组件现已可用。该实验使用高通量微卫星基因分型和半种子分析，通过分子育种策略处理四个独立杂交，将多种害虫抗性基因引入加拿大小麦。这包括两次回交和总共六个 FHB 抗性 QTL、橙花小麦吸蚊抗性 (Sm1) 和叶锈病抗性 (Lr21) 的选择。此外，用 45-76 个标记监测优良遗传背景的固定，以加速每次回交时遗传背景的恢复。该策略在 BC2F1 代中平均固定了 87% 的精英遗传背景，并成功引入了包含 FHB、Sm1 和 Lr21 抗性基因的所有染色体片段。分子育种策略在25个月内完成，与春小麦常规杂交和选择的速度相同。
Fusarium head blight of wheat is a major deterrent to wheat production world-wide. The genetics of FHB resistance in wheat are becoming clear and there is a good understanding of the genome location of FHB resistance QTL from different sources such as Sumai3, Wuhan, Nyubai and Frontana. All the components needed for assembling complex genotypes through large-scale molecular breeding experiments are now available. This experiment used high throughput microsatellite genotyping and half-seed analysis to process four independent crosses through a molecular breeding strategy to introduce multiple pest resistance genes into Canadian wheat. This included two backcrosses and selection for a total of six FHB resistance QTL, orange blossom wheat midge resistance (Sm1) and leaf rust resistance (Lr21). In addition, the fixation of the elite genetic background was monitored with 45-76 markers to accelerate restoration of the genetic background at each backcross. The strategy resulted in 87% fixation of the elite genetic background on average at the BC2F1 generation and successfully introduced all of the chromosome segments containing FHB, Sm1 and Lr21 resistance genes. The molecular breeding strategy was completed in 25 months, at an equal pace to conventional crossing and selection of spring wheat.