EARLY EYE MORPHOGENESIS IN THE ZEBRAFISH, BRACHYDANIO-RERIO
EARLY EYE MORPHOGENESIS IN THE ZEBRAFISH, BRACHYDANIO-RERIO
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DOI：
10.1002/cne.903440404
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发表时间：
1994-06-22
影响因子：
2.5
通讯作者：
DOWLING, JE
中科院分区：
文献类型：
--
作者：
SCHMITT, EA;DOWLING, JE
通过光学显微镜和扫描电子显微镜研究了受精后 12 至 36 小时内斑马鱼的早期眼睛形态发生。总体而言，斑马鱼的早期眼睛形态发生与其他脊椎动物相似，尽管视原基作为固体细胞团从前脑外溢。初始外翻后（6-7体节阶段[SS]），视原基呈现翼状形状（8-9 SS）。随后，它们向腹侧弯曲并向前轻微旋转（10-12 SS）。这些变化使原基从水平方向转变为相对于胚胎神经轴更垂直的方向。内陷从每个原基 (14 SS) 的中心开始，对称地向外周 (14-20 SS) 进展。脉络裂是由沿着眼罩前部区域的复旧形成的（18-20 SS）。受精后 24 小时 (pf)，眼罩已形成良好。在产后 24 至 36 小时之间，眼睛相对于胚胎的轴进一步旋转，这将脉络组织在产后 36 小时重新定位到典型的腹侧位置。由于早期形态发生过程中眼睛的两次旋转，特别是后来的一次，新兴视原基的前后方向最终成为完整眼杯的腹背轴。 (C) 1994 威利利斯公司
Early eye morphogenesis in the zebrafish between 12 and 36 hours postfertilization was studied by light- and scanning electron microscopy. Overall, early eye morphogenesis in the zebrafish is similar to that of other vertebrates even though the optic primordia evaginate from the forebrain as solid masses of cells. After initial evagination (6-7 somite stage [SS]), the optic primordia take on a wing-like shape (8-9 SS). Subsequently, they bend ventrally and rotate slightly in an anterior direction (10-12 SS). These changes serve to bring the primordia from a horizontal to a more vertical orientation in relation to the embryonic neural axis. Invagination commences from the center of each primordium (14 SS) and progresses symmetrically out towards the periphery (14-20 SS). The choroid fissure forms by an involution along the anterior region of the eyecup (18-20 SS). By 24 hours postfertilization (pf) the eyecups are well formed. Between 24 and 36 hours pf, the eyes rotate further in relation to the axis of the embryo, and this repositions the choroid fissue to a typical ventral location by 36 hours pf. Because of the two rotations of the eye during early morphogenesis, particularly the later one, the anterior-posterior orientation of the emerging optic primordium ultimately becomes the ventral-dorsalaxis of the completed eyecup. (C) 1994 WiIey-Liss, Inc.