Colocalization of synapsin and actin during synaptic vesicle recycling.
Colocalization of synapsin and actin during synaptic vesicle recycling.
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DOI：
10.1083/jcb.200212140
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发表时间：
2003-05-26
期刊：
影响因子：
--
通讯作者：
Shupliakov O
中科院分区：
文献类型：
--
作者：
Bloom O;Evergren E;Tomilin N;Kjaerulff O;Löw P;Brodin L;Pieribone VA;Greengard P;Shupliakov O
据推测，在成熟的神经末梢中，突触蛋白和肌动蛋白之间的相互作用调节突触小泡的聚集以及突触活动期间小泡释放的可用性。在这里，我们使用免疫金电子显微镜来检查不同突触活动状态下七鳃鳗巨型突触中肌动蛋白和突触蛋白的亚细胞定位。与早期的观察结果一致，在静止的突触中，突触蛋白免疫反应性优先定位于活性区远端的囊泡簇的一部分。然而，在突触活动期间，在靠近活动区的囊泡池中检测到突触蛋白。此外，肌动蛋白和突触蛋白被发现共定位于突触小泡回收位点、内吞区的动态丝状细胞基质中。突触蛋白免疫标记与网格蛋白包被的中间体无关，但在似乎循环回到簇的囊泡上发现。通过显微注射抗突触蛋白抗体破坏突触蛋白功能，导致活性突触内吞区的细胞基质显着减少。我们的数据表明，除了其已知的在储备池中囊泡聚集的功能之外，突触蛋白在突触活动期间从突触囊泡簇迁移并参与内吞区富含肌动蛋白的细胞基质的组织。
It has been hypothesized that in the mature nerve terminal, interactions between synapsin and actin regulate the clustering of synaptic vesicles and the availability of vesicles for release during synaptic activity. Here, we have used immunogold electron microscopy to examine the subcellular localization of actin and synapsin in the giant synapse in lamprey at different states of synaptic activity. In agreement with earlier observations, in synapses at rest, synapsin immunoreactivity was preferentially localized to a portion of the vesicle cluster distal to the active zone. During synaptic activity, however, synapsin was detected in the pool of vesicles proximal to the active zone. In addition, actin and synapsin were found colocalized in a dynamic filamentous cytomatrix at the sites of synaptic vesicle recycling, endocytic zones. Synapsin immunolabeling was not associated with clathrin-coated intermediates but was found on vesicles that appeared to be recycling back to the cluster. Disruption of synapsin function by microinjection of antisynapsin antibodies resulted in a prominent reduction of the cytomatrix at endocytic zones of active synapses. Our data suggest that in addition to its known function in clustering of vesicles in the reserve pool, synapsin migrates from the synaptic vesicle cluster and participates in the organization of the actin-rich cytomatrix in the endocytic zone during synaptic activity.
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影响因子：
5.5
作者：
LLINAS, R;GRUNER, JA;GREENGARD, P
通讯作者：
GREENGARD, P
DOI：
10.1073/pnas.93.8.3679
发表时间：
1996-04-16
影响因子：
11.1
作者：
Jovanovic, JN;Benfenati, F;Czernik, AJ
通讯作者：
Czernik, AJ
DOI：
10.1098/rstb.1999.0378
发表时间：
1999-02-28
期刊：
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
Hilfiker, S;Pieribone, VA;Greengard, P
通讯作者：
Greengard, P
影响因子：
2.5
作者：
BRODIN, L;SHUPLIAKOV, O;HILL, RH
通讯作者：
HILL, RH
影响因子：
16.2
作者：
BENFENATI, F;VALTORTA, F;GREENGARD, P
通讯作者：
GREENGARD, P