Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens
Validation of a new multiplex assay against individual immunoassays for the quantification of reproductive, stress, and energetic metabolism biomarkers in urine specimens
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DOI：
10.1002/ajhb.21229
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发表时间：
2012-01-01
影响因子：
2.9
通讯作者：
Nepomnaschy, Pablo A.
中科院分区：
文献类型：
--
作者：
Salvante, Katrina G.;Brindle, Eleanor;Nepomnaschy, Pablo A.
在一次检测中同时测量多种激素可以节省样品体积、劳动力、时间、试剂、金钱和耗材。因此，多重阵列代表了一个更快，更经济和生态上合理的替代singleton assays.Objectives：为了验证一个新的，市售的多重女性阵列生产的Quansys Biosciences对个人免疫测定的定量六种激素的尿液样本中的妇女在不同的生殖阶段。使用新的Quansys多重女性激素阵列分析尿样，并与脂联素、游离皮质醇、c肽、雌酮-3-葡萄糖醛酸苷（E1 G）促卵泡激素β-亚单位（FSH-β）和人绒毛膜促性腺激素β-亚单位（hCG-β）。使用Pearson相关性、线性回归和Bland-Altman分析评估测定之间的相关性。游离皮质醇、E1 G、FSH-β和hCG-β的时间曲线也进行了比较。结果：多重阵列与五种测试激素的个体免疫测定高度相关（Pearson相关系数>= 0.75），并产生与游离皮质醇、E1 G、FSH-β和hCG-β的个体免疫测定一致的激素谱的时间模式。Quansys多重雌性激素阵列是个体免疫测定的有效替代方法，用于定量人尿样中的应激、生殖和能量激素和代谢物，并可用于检查这些激素之间的动态相互作用。Am. J.哈姆。24：81-86，2012. (C)2011Wiley Periodicals，Inc.
Measuring multiple hormones simultaneously in a single assay saves sample volume, labor, time, reagents, money, and consumables. Thus, multiplex arrays represent a faster, more economically and ecologically sound alternative to singleton assays.Objectives: To validate a new, commercially available multiplex female array produced by Quansys Biosciences against individual immunoassays for the quantification of six hormones in urine samples from women in different reproductive stages.Methods: Urine samples were analyzed using the new Quansys multiplex female hormone array and compared with well-established individual immunoassays for adiponectin, free cortisol, c-peptide, estrone-3-glucuronide (E1G), follicle stimulating hormone beta-subunit (FSH-beta), and human chorionic gonadotropin beta-subunit (hCG-beta). Correlations between assays were assessed using Pearson correlation, linear regression and Bland-Altman analysis. The temporal profiles of free cortisol, E1G, FSH-beta, and hCG-beta were also compared.Results: The multiplex array was highly correlated with the individual immunoassays for five of the tested hormones (Pearson's correlation coefficient >= 0.75), and yielded temporal patterns of hormone profiles consistent with the individual immunoassays for free cortisol, E1G, FSH-beta, and hCG-beta.Conclusions: The Quansys multiplex female hormone array is a valid alternative method to individual immunoassays for the quantification of stress, reproductive and energetic hormones and metabolites in human urine samples and can be used to examine the dynamic interactions between these hormones. Am. J. Hum. Biol. 24: 81-86, 2012. (C) 2011Wiley Periodicals, Inc.