Towards the understanding of hyperthermophilic methanogenesis from waste activated sludge at 70 degrees C: Performance, stability, kinetic and microbial community analyses
Towards the understanding of hyperthermophilic methanogenesis from waste activated sludge at 70 degrees C: Performance, stability, kinetic and microbial community analyses
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了解 70 摄氏度下废弃活性污泥的超高温产甲烷作用：性能、稳定性、动力学和微生物群落分析
DOI：
10.1016/j.wasman.2021.02.037
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发表时间：
2021
期刊：
影响因子：
8.1
通讯作者：
Lyu Yong-Kang
中科院分区：
文献类型：
--
作者：
Wu Li-Jie;Li Xiao-Xiao;Zhou Quan;Yang Fan;Ren Rui-Peng;Lyu Yong-Kang
厌氧消化是降解废弃活性污泥的一种很有前途的方法。然而，传统的工艺通常停留在有限的水解和糟糕的病原体破坏上。在70摄氏度下的高温消化在能源需求相对较低和操作困难的情况下克服这些问题引起了人们的注意。为了阐明其运行特性，将单级高温消化池控制在70℃，并连续运行以降解IS。在高温体系中，甲烷产率可达88.7g/g VS，是中温体系的4倍。动力学分析表明，高温消化有利于非降解物的转化。因此，在超高温条件下的水解率能够得到显著提高。超高温系统获得如此高的甲烷产量需要超过10d。在稳定性方面，有机负荷高于10.2ug vs/L/d，导致产甲烷和丙酸、丁酸和戊酸积累的限制增加。在高温系统中，除产甲烷的优势种甲硫菊酯外，还有两种水生型methanogensMethanospirillumandMethanothermobacterreached，分别为18.84%和8.31%。酷热杆菌属隶属于微生物门，在高温消化池中对蛋白质的降解有较大贡献。
Anaerobic digestion is promising for waste activated sludge (WAS) degradation. However, conventional processes were generally stuck with limited hydrolysis and poor pathogen destruction. Hyperthermophilic digestion at 70 °C has drawn attention in overcoming those issues at a relatively low energy requirement and operating difficulties. In order to illuminate its operation characteristics, a single-stage hyperthermophilic digester was controlled at 70 °C and operated continuously to degrade WAS. 88.7 mL/g VSaddedof methane yield could be achieved in the hyperthermophilic system, fourfold higher than that in the mesophilic system. Kinetic analysis revealed that hyperthermophilic digestion was advantageous in converting the non-degradable fraction. Consequently, hydrolysis under the hyperthermophilic condition was able to be significantly improved. Above 10 d was necessary for the hyperthermophilic system to gain such a high methane production. In the case of stability, the organic loading of higher than 10.2 g VS/L/d resulted in increasing limitation from methanogenesis and accumulation of propionic, butyric and valeric acids. In addition to the dominant acetoclastic genusMethanothrixfor methane production in the hyperthermophilic system, two hydrogenotrophic methanogensMethanospirillumandMethanothermobacterreached 18.84% and 8.31%, respectively. The genusCoprothermobacter, affiliated with the phylumFirmicutes, made more contribution to protein hydrolysis in the hyperthermophilic digester.