Different Distributions of Epstein-Barr Virus Early and Late Gene Transcripts within Viral Replication Compartments
Different Distributions of Epstein-Barr Virus Early and Late Gene Transcripts within Viral Replication Compartments
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DOI：
10.1128/jvi.00219-13
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发表时间：
2013-06-01
影响因子：
5.4
通讯作者：
Tsurumi, Tatsuya
中科院分区：
文献类型：
--
作者：
Sugimoto, Atsuko;Sato, Yoshitaka;Tsurumi, Tatsuya
EB病毒（EBV）的生产性复制发生在细胞核中的离散位点，称为复制区室，其中发生病毒基因组DNA合成和转录。复制区室包括亚核结构域，称为BMRF 1核心，其高度富集BMRF 1蛋白。在病毒裂解复制过程中，新合成的病毒DNA基因组围绕BMRF 1核心组织，然后储存在BMRF 1核心内。在这里，我们研究了空间分布的病毒早期和晚期基因mRNA的复制隔间内使用共聚焦激光扫描显微镜和三维表面重建成像。EBV早期mRNA主要位于BMRF1核心的外部，而病毒晚期mRNA则位于内部，这与晚期基因转录依赖于病毒DNA复制的事实相一致。从这些结果，我们推测，网站的病毒早期和晚期基因转录是分开的参考BMRF1核心。
Productive replication of the Epstein-Barr virus (EBV) occurs in discrete sites in nuclei, called replication compartments, where viral genome DNA synthesis and transcription take place. The replication compartments include subnuclear domains, designated BMRF1 cores, which are highly enriched in the BMRF1 protein. During viral lytic replication, newly synthesized viral DNA genomes are organized around and then stored inside BMRF1 cores. Here, we examined spatial distribution of viral early and late gene mRNAs within replication compartments using confocal laser scanning microscopy and three-dimensional surface reconstruction imaging. EBV early mRNAs were mainly located outside the BMRF1 cores, while viral late mRNAs were identified inside, corresponding well with the fact that late gene transcription is dependent on viral DNA replication. From these results, we speculate that sites for viral early and late gene transcription are separated with reference to BMRF1 cores.