microRNA expression profiling of heart tissue during fetal development.
microRNA expression profiling of heart tissue during fetal development.
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DOI：
10.3892/ijmm.2014.1691
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发表时间：
2014-05
影响因子：
5.4
通讯作者：
Jizi Zhou;Xinran Dong;Qiongjie Zhou;Huijun Wang;Yanyan Qian;Weidong Tian;Duan Ma;Xiaotian Li
中科院分区：
文献类型：
--
作者：
Jizi Zhou;Xinran Dong;Qiongjie Zhou;Huijun Wang;Yanyan Qian;Weidong Tian;Duan Ma;Xiaotian Li
microRNAs（miRNAs）在心脏发育早期和心脏成熟过程中均起重要作用。本研究的目的是确定miRNAs在人胎儿心脏中的阶段特异性表达，以确定有价值的目标，为进一步研究心脏缺陷。使用Affyssin微阵列获得来自妊娠5、7、9和23周的人胎儿心脏组织的miRNA表达谱。为了鉴定在每个时间点差异表达的miRNA，采用通过R limma算法的线性回归分析。用Cluster3.0软件进行系统聚类分析。对来自不同簇的miRNA进行基因本体论分析。确定了miRNA家族和基因组定位的共性，并通过定量聚合酶链反应（qPCR）验证了来自不同簇的所选miRNA的差异表达。在人胎心组织中共检测到703个miRNAs。其中，288个差异表达的miRNAs代表5个具有不同表达趋势的簇。几个成簇的miRNA也在miRNA家族或近端基因组定位内共享分类。qPCR证实了所选miRNA的表达模式。预测5个簇中的miRNA靶向对心脏发育至关重要的基因，并参与影响心脏结构形成和心脏相关细胞事件的细胞信号传导途径。总之，据我们所知，这是第一个人胎儿心脏组织的miRNA表达谱研究。特异性miRNAs的阶段特异性表达表明在胎儿心脏发育过程中不同时间点的潜在作用。
microRNAs (miRNAs) are important both in early cardiogenesis and in the process of heart maturation. The aim of this study was to determine the stage-specific expression of miRNAs in human fetal heart in order to identify valuable targets for further study of heart defects. Affymetrix microarrays were used to obtain miRNA expression profiles from human fetal heart tissue at 5, 7, 9 and 23 weeks of gestation. To identify differentially expressed miRNAs at each time-point, linear regression analysis by the R limma algorithm was employed. Hierarchical clustering analysis was conducted with Cluster 3.0 software. Gene Ontology analysis was carried out for miRNAs from different clusters. Commonalities in miRNA families and genomic localization were identified, and the differential expression of selected miRNAs from different clusters was verified by quantitative polymerase chain reaction (qPCR). A total of 703 miRNAs were expressed in human fetal heart. Of these, 288 differentially expressed miRNAs represented 5 clusters with different expression trends. Several clustered miRNAs also shared classification within miRNA families or proximal genomic localization. qPCR confirmed the expression patterns of selected miRNAs. miRNAs within the 5 clusters were predicted to target genes vital for heart development and to be involved in cellular signaling pathways that affect heart structure formation and heart-associated cellular events. In conclusion, to the best of our knowledge, this is the first miRNA expression profiling study of human fetal heart tissue. The stage-specific expression of specific miRNAs suggests potential roles at distinct time-points during fetal heart development.