Concomitant enhancement of a cytoskeleton-associated 76,000-dalton protein and inhibition of fluid-phase pinocytosis by interferon-alpha in Fujinami sarcoma virus-transformed rat 3Y1 cells.
Concomitant enhancement of a cytoskeleton-associated 76,000-dalton protein and inhibition of fluid-phase pinocytosis by interferon-alpha in Fujinami sarcoma virus-transformed rat 3Y1 cells.
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DOI：
10.1089/jir.1986.6.563
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发表时间：
1986-10
期刊：
影响因子：
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通讯作者：
S. Lin;H. Schellekens;I. Tamm
中科院分区：
文献类型：
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作者：
S. Lin;H. Schellekens;I. Tamm
向Fujinami肉瘤病毒转化的大鼠3 Y1细胞中加入大鼠干扰素-α（IFN-α）可逐渐抑制液相胞饮作用[3 h时抑制10%; 12 h时抑制最大（60%）]。从暴露于IFN 24小时的培养物的细胞骨架部分的电泳分析揭示了一种新的76，000-道尔顿蛋白（CKp 76）。其出现的动力学与抑制液相胞饮作用有关。在加入IFN前，用放线菌素D预处理或用[35 S]甲硫氨酸预标记的培养物中未检测到CKp 76。然而，放线菌酮的存在下，在孵育过程中与IFN有没有影响CKp 76的合成后，删除这两个代理。这些结果表明，CKp 76的出现是由于其基因的转录增强响应IFN。亚细胞分级显示诱导的CKp 76在核团中的存在。从这些结果来看，CKp 76可能至少部分地负责IFN对液相胞饮作用的影响。
Addition of rat interferon-alpha (IFN-alpha) to Fujinami sarcoma virus-transformed rat 3Y1 cells progressively inhibited fluid-phase pinocytosis [10% inhibition at 3 h; maximal (60%) inhibition by 12 h]. Electrophoretic analysis of the cytoskeletal fraction from cultures exposed to IFN for 24 h revealed a novel 76,000-dalton protein (CKp76). The kinetics of its appearance paralleled the inhibition of fluid-phase pinocytosis. CKp76 was not detected in cultures pretreated with actinomycin D, or prelabeled with [35S]methionine, prior to IFN addition. However, the presence of cycloheximide during incubation with IFN had no effect on the synthesis of CKp76 after removal of both agents. These results suggest that the appearance of CKp76 was due to enhanced transcription of its gene in response to IFN. Subcellular fractionation revealed the presence of induced CKp76 in the nuclear pellet. From these results it is possible that CKp76 may be responsible at leat in part for the effects of IFN on fluid-phase pinocytosis.