Transient expression of a chondroitin sulfate-related epitope during cartilage histomorphogenesis in the axial skeleton of fetal rats.
Transient expression of a chondroitin sulfate-related epitope during cartilage histomorphogenesis in the axial skeleton of fetal rats.
复制标题
胎鼠中轴骨骼软骨组织形态发生过程中硫酸软骨素相关表位的瞬时表达。
DOI：
10.1016/0012-1606(89)90051-1
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发表时间：
1989
影响因子：
2.7
通讯作者：
Ruch,JV
中科院分区：
文献类型：
--
作者：
Mark,MP;Butler,WT;Ruch,JV
在小鼠中针对骨蛋白混合物产生的单克隆抗体 (MC21C) 被发现对天然硫酸软骨素链表现出独特的反应性。对胎鼠发育不同阶段的组织切片进行间接免疫荧光和免疫过氧化物酶检测，以研究 MC21C 表位在软骨形态发生和分化过程中的分布。这种细胞外标记存在于巩膜切开器中，其分布随后遵循软骨前脊柱的分割模式。此外，MC21C 免疫染色模式的变化与椎骨的初始生长密切相关。在中轴骨骼（脊柱、颅底）中，MC21C 的免疫染色在软骨前凝结中最大，然后在软骨化过程中迅速消失。此外，突索周围基质被强烈免疫染色。
A monoclonal antibody (MC21C), raised in mouse in response to a mixture of bone proteins, was found to exhibit a unique reactivity toward native chondroitin sulfate chains. Indirect immunofluorescence and immunoperoxidase assays were performed on tissue sections at different stages of fetal rat development, in order to investigate the distribution of the MC21C epitope during cartilage morphogenesis and differentiation. This extracellular marker was present in the sclerotome and its distribution subsequently followed the segmentation pattern of the precartilaginous vertebral column. In addition, changes in the MC21C-immunostaining pattern strongly correlated with the initial growth of the vertebrae. In the axial skeleton (spinal column, basis cranii), the immunostaining by MC21C was maximum in precartilaginous condensations and then rapidly disappeared during the process of chondrification. Also, the perinotochordal matrix was intensely immunostained.