INTERLEUKIN-4 AND INTERFERON-GAMMA PRODUCTION IN RESTIMULATED CD4+ AND CD8+ CELLS INDICATES MEMORY TYPE RESPONSIVENESS
INTERLEUKIN-4 AND INTERFERON-GAMMA PRODUCTION IN RESTIMULATED CD4+ AND CD8+ CELLS INDICATES MEMORY TYPE RESPONSIVENESS
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DOI：
10.1111/j.1365-3083.1991.tb01774.x
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发表时间：
1991-03-01
影响因子：
3.7
通讯作者：
MOLLER, E
中科院分区：
文献类型：
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作者：
SANDER, B;CARDELL, S;MOLLER, E
白细胞介素4（IL-4）和干扰素γ（IFN-γ）的生产进行了分析，在小鼠脾细胞在体外的初级和次级有丝分裂原刺激。 结合胞浆内免疫荧光和表面染色研究单个淋巴因子产生细胞的动力学、频率和表型。 IL-4和IFN-γ均由CD 4+和CD 8+细胞产生，但75-80%的IL-4产生者为CD 4+，而90%的IFN-γ产生者为CD 8+。 两种刺激程序诱导的IFN-γ +细胞的频率大致相同，但动力学不同，使用Con A在30 h时达到峰值，使用抗CD 3和PMA在52 h时达到峰值。 IL-4的产生在52 h达到高峰，但经抗CD 3和PMA刺激后，IL-4+细胞的频率比ConA刺激后高8-10倍，在丝裂原激活的细胞再刺激过程中，淋巴因子迅速产生，IL-4和IFN-γ的产生在8-11 h达到高峰。 仅观察到IL-4+细胞频率的小幅增加，最多增加2至3倍。 没有证据表明初级和次级刺激之间产生的淋巴因子发生了重大转变，相反，初级刺激诱导的淋巴因子产生模式在次级培养中也占主导地位，与刺激条件无关。
Interleukin 4 (IL-4) and interferon gamma (IFN-gamma) production was analysed in murine spleen cells during primary and secondary mitogen stimulation in vitro. The kinetics, frequency and phenotype of single lymphokine-producing cells were studied by combining intracytoplasmatic immunofluorescence and surface staining. Both IL-4 and IFN-gamma was produced by CD4+ as well as CD8+ cells, however 75-80% of IL-4 producers were CD4+ and 90% of IFN-gamma+ cells were CD8+.In primary stimulations, concanavalin A (Con A) activation or anti-CD3 antibody together with phorbol 12-myristate 13-acetate (PMA) induced different patterns of lymphokine production. Approximately the same frequency of IFN-gamma+ cells was induced by both stimulation procedures but the kinetics was different with a peak at 30 h using Con A and at 52 h using anti-CD3 and PMA. IL-4 production peaked at 52 h, but the frequency of IL-4+ cells was 8-10 times higher after stimulation by anti-CD3 and PMA than after Con A stimulation.During restimulation of the mitogen activated cells, lymphokines were rapidly produced; both IL-4 and IFN-gamma production peaked at 8-11 h. Only a small increase in the frequency of IL-4+ cells was seen, at most two to three times. No evidence for a major shift of lymphokines produced between primary and secondary stimulations could be found. Instead, the pattern of lymphokine production induced by the primary stimulus was dominant also in secondary cultures irrespective of stimulation condition.