Nuclei of stroma: Site of highest estrogen concentration in human benign prostatic hyperplasia
Nuclei of stroma: Site of highest estrogen concentration in human benign prostatic hyperplasia
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基质核：人类良性前列腺增生中雌激素浓度最高的部位
DOI：
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发表时间：
1982
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通讯作者：
K. Voigt
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作者：
Ivan Kozák;W. Bartsch;M. Krieg;K. Voigt
在早期的出版物中，我们发现与人类良性前列腺增生（BPH）的上皮相比，基质中的雌激素受体阳性病例显著更多。因此，我们感兴趣的是，发现这种在基质中的雌激素受体的优先测定是否反映了该组织部分中较高的雌激素含量。用放射免疫分析法测定了整个BPH组织、间质和上皮以及间质和上皮细胞核部分中的雌激素（E2）和雌酮（E1）。为了比较，还在血液中测量E2和E1。主要结果如下：1）在所有BPH样本中，E2（26.0 ± 3.5（SEM）pg/g（n = 20））和E2（53.5 ± 7.4（14））显著高于相应血浆E2：17.5 ± 2.3pg/ml（11例），E1：26.5 ± 4.4（7例），E2/E1比值分别为0.52和0.69; 2）在基质中E2显著增加（53.6 ± 7.8 fmol/mg DNA（18））和E1，（55.9 ± 8.4（12））例，（E2：15.7 ± 1.9（16）; 1：20.7 ± 3.2（13））; 3）在间质细胞核中E2显著升高（38.0 ± 5.3 fmol/mg DNA（20））和E1（19.2 ± 4.8（14））个浓度高于上皮细胞（E2：6.8 ± 3.1（19）; E1：2.5 ± 0.5（13）），间质和上皮细胞核E2/ E1比值分别为2.3和2.7，均显著高于正常前列腺组织。总之，有一个明显的积累雌激素的细胞核中的基质，E2浓度显着高于E1。这些数据支持了E2在刺激BPH间质生长中可能发挥优先作用的假设。
In an earlier publication we found significantly more estrogen receptor‐positive cases in the stroma if compared with the epithelium of human benign prostatic hyperplasia (BPH). We were therefore interested to find out whether this preferential assay of estrogen receptor in stroma is reflected by a higher estrogen content in this tissue fraction. Estradiol (E2) and estrone (E1) were measured by RIA in whole BPH tissue, stroma, and epithelium as well as in the nuclear fraction of stroma and epithelium. For comparison, E2 and E1 were measured in blood also. The main results are as follows: 1) In all BPH samples the E2 (26.0 ± 3.5(SEM) pg/g (n = 20)) and E2 (53.5 ± 7.4 (14)) content was significantly higher than in the corresponding plasma (E2: 17.5 ± 2.3 pg/ml (11); E1: 26.5 ± 4.4 (7)), the mean ratio of E2/E1, was 0.52 and 0.69 for BPH and plasma, respectively; 2) in stroma significantly more E2 (53.6 ± 7.8 fmol/mg DNA (18)) and E1, (55.9 ± 8.4 (12)) were measured than in epithelium (E2: 15.7 ± 1.9(16); 1: 20.7 ± 3.2(13)); 3) in nuclei of stroma significantly higher E2 (38.0 ± 5.3 fmol/mg DNA (20)) and E1 (19.2 ± 4.8 (14)) concentrations were present than in epithelium (E2: 6.8 ± 3.1 (19); E1: 2.5 ± 0.5 (13)), the mean ratio of E2/ E1 for thenuclear fractions of stroma (2.3) and epithelium (2.7) increased dramatically if compared with the respective ratio in whole BPH tissue. In conclusion, there is a distinct accumulation of estrogens in the nuclei of stroma, E2 concentration being significantly higher than E1. These data support the hypothesis that E2 could play a preferential role in stimulating the growth of the BPH stroma.