A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression
A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression
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一种新的长链非编码RNA——DIR，通过改变胁迫相关基因的表达来提高木薯的耐旱性
DOI：
10.1016/j.jia.2022.07.022
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发表时间：
2022-08-19
影响因子：
4.8
通讯作者：
Peng Ming
中科院分区：
文献类型：
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作者：
Dong Shi-man;Xiao Liang;Peng Ming
木薯是重要的热带经济作物。严重的干旱胁迫影响木薯的产量和品质，给农业生产造成巨大的经济损失。提高木薯的耐旱性可以有效地提高其产量。长链非编码RNA（lncRNA）存在于多种真核生物中。近年来，越来越多的证据表明lncRNA在植物对非生物胁迫的应答中起着重要作用。然而，木薯lncRNA在干旱反应中的功能在很大程度上仍然未知。在这项研究中，我们鉴定了一种新的lncRNA，干旱诱导的基因间lncRNA（DROUGHT-INDUCED INTEGENIC lncRNA，DINCRNA）。基因表达分析表明，干旱胁迫能显著诱导拟南芥的萌发，而脱落酸（阿坝）和茉莉酸（JA）处理对拟南芥的萌发无明显影响。此外，过表达的p53基因增强了转基因木薯中脯氨酸的积累和耐旱性。RNA-seq分析显示，干旱优先影响与转录和代谢相关的干旱相关基因。此外，RNA下拉质谱分析表明，与325个蛋白质的相互作用，cDNA 3。蛋白质-蛋白质相互作用（PPI）分析发现与mRNA输出和蛋白质质量控制途径相关的蛋白质显著富集。总的来说，这些结果表明，拟南芥及其相互作用的蛋白质，调节mRNA或蛋白质代谢参与介导干旱胁迫反应。因此，调节case 3的表达具有提高干旱条件下木薯产量的潜力。
Cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs (lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA (DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid (ABA) or jasmonic acid (JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein-protein interaction (PPI) analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control pathways. Collectively, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.