Nuclear transplantation in different strains of zebrafish
Nuclear transplantation in different strains of zebrafish
复制标题
不同品系斑马鱼的核移植
DOI：
10.1360/02tb9283
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发表时间：
2002-08-01
影响因子：
--
通讯作者：
Zhu, ZY
中科院分区：
文献类型：
--
作者：
Hu, W;Wang, YP;Zhu, ZY
将AB系斑马鱼单个晚期囊胚细胞核移植到长鳍系去核未受精卵中。在1119个克隆胚中，14个重组胚发育成鱼苗。克隆鱼的DNA指纹图谱与核供体鱼的DNA指纹图谱相似，但与核受体鱼的DNA指纹图谱明显不同。证实了遗传物质来源于核供体细胞而非核受体卵子。这项研究表明，用不同品系的斑马鱼进行核移植的基本技术已经取得了突破。这对研究基因功能、动物发育过程中基因表达的调控、细胞核的发育潜能以及供体细胞核与受体细胞质之间的相互作用等重要发育问题具有重要意义。
Single later blastula nuclei from AB strain of zebrafish (Danio rerio) were transplanted into enucleated unfertilized eggs of Long fin strain. Of 1119 cloning embryos, 14 reconstructed embryos developed into fry. DNA fingerprinting systems of the cloned fish were similar to those of the nuclear donor fish, but were distinctly different from those of the nuclear recipient fish. It confirmed that the genetic material originated from nuclear donor cell other than from nuclear recipient egg. The research suggested that the basic technique for nuclear transplantation performed with different strains of zebrafish has made a breakthrough. It should be helpful for the study of some important developmental problems such as gene function, the regulation ogene expression during animal development, the developmental potential of a nucleus and the interactions between the donor nucleus and the recipient cytoplasm, etc.