Expression of fibroblast growth factor-2 and brain-derived neurotrophic factor mRNA in the medial prefrontal cortex and hippocampus after uncontrollable or controllable stress
Expression of fibroblast growth factor-2 and brain-derived neurotrophic factor mRNA in the medial prefrontal cortex and hippocampus after uncontrollable or controllable stress
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DOI：
10.1016/j.neuroscience.2006.11.026
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发表时间：
2007-02-23
期刊：
影响因子：
3.3
通讯作者：
Maier, S. F.
中科院分区：
文献类型：
--
作者：
Bland, S. T.;Tamlyn, J. P.;Maier, S. F.
已知神经营养因子，包括碱性成纤维细胞生长因子（FGF-2）和脑源性神经营养因子（BDNF），受到暴露于压力经历的影响。在这里，我们研究的影响行为可控（逃避tailshock，ES）或不可控（不可避免的tailshock，IS）的压力对FGF-2和BDNF mRNA的表达在内侧前额叶皮层（mPFC）和海马结构（HF）的雄性SD大鼠的子区域。将ES大鼠放置在配备有自由旋转轮的树脂玻璃盒中，将IS大鼠放置在轮子固定的相同盒中。将ES和IS大鼠用轭连接，使它们接受相同的尾部电击，但ES大鼠可以终止两种大鼠的每次电击。没有应激对照（NS）留在其饲养笼中。在应激结束后0、2、24或72 h处死大鼠。原位杂交检测mPFC和HF中FGF-2和BDNF mRNA的表达。在mPFC中，ES在应激后0和2 h产生FGF-2 mRNA表达的显著增加。在HF中，ES仅在CA 2中产生比IS和NS更大的FGF-2 mRNA表达增加。ES还引起应激后0 h前扣带回BDNF mRNA表达增加。在HF中没有观察到应激源可控性对BDNF的影响，尽管ES和IS都降低了DG中的BDNF mRNA。mPFC中的FGF-2可能参与压力体验期间的情绪调节（“应对”）。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Neurotrophic factors, including basic fibroblast growth factor (FGF-2) and brain-derived neurotrophic factor (BDNF) are known to be affected by exposure to stressful experiences. Here, we examine the effects of behaviorally controllable (escapable tailshock, ES) or uncontrollable (inescapable tailshock, IS) stress on the expression of FGF-2 and BDNF mRNA in subregions of the medial prefrontal cortex (mPFC) and the hippocampal formation (HF) of male Sprague-Dawley rats. ES rats were placed in Plexiglas boxes equipped with a free spinning wheel and IS rats were placed in identical boxes with the wheels fixed. ES and IS rats were yoked such that they received the same tailshocks, but the ES rat could terminate each shock for both rats. No stress controls (NS) remained in their home cages. Rats were killed 0, 2, 24, or 72 h after termination of the stress session. In situ hybridization was performed to measure FGF-2 and BDNF mRNA in the mPFC and HF. In the mPFC, ES produced a significant increase in FGF-2 mRNA expression at 0 and 2 h post-stress. In the HF, ES produced a greater increase in FGF-2 mRNA expression than IS and NS only in CA2. ES also produced an increase in BDNF mRNA expression in the anterior cingulate at 0 h post-stress. No effects of stressor controllability on BDNF were observed in the HF, although both ES and IS decreased BDNF mRNA in the DG. FGF-2 in the mPFC may be involved in emotional regulation ("coping") during stressful experiences. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.