Imaging mass spectrometry demonstrates age-related decline in human adipose plasticity
Imaging mass spectrometry demonstrates age-related decline in human adipose plasticity
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DOI：
10.1172/jci.insight.90349
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发表时间：
2017-03-09
期刊：
影响因子：
8
通讯作者：
Steinhauser, Matthew L.
中科院分区：
文献类型：
--
作者：
Guillermier, Christelle;Fazeli, Pouneh K.;Steinhauser, Matthew L.
稳定同位素示踪剂的量化揭示了活组织的动态状态。一种新形式的成像质谱法可以量化远小于立方微米的区域的同位素比率，从而可以在单细胞水平上使用稳定的同位素示踪剂测量细胞的更新和代谢，我们称之为多同位素成像质谱法。在首次人体研究中，我们利用DNA合成和新生脂肪生成的稳定同位素示踪剂来前瞻性地测量细胞出生和脂肪细胞脂质周转。在一项健康成人的研究中，我们阐明了新脂肪细胞生成和脂肪细胞脂质周转的年龄依赖性下降。线性回归模型表明，衰老效应可能是由胰岛素样生长因子-1 （IGF-1）的下降介导的。因此，本研究建立了一种测量人体细胞更新和代谢的亚细胞分辨率方法，同时暗示生长激素/IGF-1轴与脂肪组织老化有关。
Quantification of stable isotope tracers has revealed the dynamic state of living tissues. A new form of imaging mass spectrometry quantifies isotope ratios in domains much smaller than a cubic micron, enabling measurement of cell turnover and metabolism with stable isotope tracers at the single-cell level with a methodology we refer to as multi-isotope imaging mass spectrometry. In a first-in-human study, we utilize stable isotope tracers of DNA synthesis and de novo lipogenesis to prospectively measure cell birth and adipocyte lipid turnover. In a study of healthy adults, we elucidate an age-dependent decline in new adipocyte generation and adipocyte lipid turnover. A linear regression model suggests that the aging effect could be mediated by a decline in insulin-like growth factor-1 (IGF-1). This study therefore establishes a method for measurement of cell turnover and metabolism in humans with subcellular resolution while implicating the growth hormone/IGF-1 axis in adipose tissue aging.