Temporal Proteomic Analysis Reveals Continuous Impairment of Intestinal Development in Neonatal Piglets with Intrauterine Growth Restriction
Temporal Proteomic Analysis Reveals Continuous Impairment of Intestinal Development in Neonatal Piglets with Intrauterine Growth Restriction
复制标题
时间蛋白质组分析揭示宫内生长受限的新生仔猪肠道发育持续受损
DOI：
10.1021/pr900747d
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Wang, Junjun
中科院分区：
文献类型：
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作者：
Wang, Xiaoqiu;Wu, Weizong;Wang, Junjun
与正常出生体重（NBW）的新生儿相比，宫内生长受限（IUGR）新生儿的营养物质利用效率降低。然而，潜在的机制在很大程度上是未知的。本研究采用时间蛋白质组学方法，结合组织学和生化分析，研究IUGR仔猪在哺乳期（第1、7和21天）小肠黏膜蛋白质组的动态变化。我们在IUGR和NBW仔猪之间鉴定了56个差异表达的蛋白点。这些蛋白质参与关键的生物过程，包括：(1)营养物质的吸收、消化和运输；(2)细胞结构和运动；(3)葡萄糖和能量代谢；(4)脂质代谢；(5)氨基酸代谢；(6)矿物质和维生素代谢；(7)细胞氧化还原稳态；(8)应激反应；(9)细胞凋亡。我们的时间蛋白质组学分析结果显示IUGR新生儿仔猪肠道发育持续受损。这些发现对于理解IUGR新生儿小肠代谢缺陷具有重要意义，并有望为改善其生存和生长提供新的策略。
Efficiency of nutrient utilization is reduced in neonates with intrauterine growth restriction (IUGR) compared with those with a normal birth weight (NBW). However, the underlying mechanisms are largely unknown. In this study, we applied temporal proteomic approach, coupled with histological and biochemical analyses, to study dynamic changes of the proteome in the small intestinal mucosa of IUGR piglets during the nursing period (Days 1, 7 and 21). We identified 56 differentially expressed protein spots between IUGR and NBW piglets. These proteins participate in key biological processes, including (1) absorption, digestion and transport of nutrients; (2) cell structure and motility; (3) glucose and energy metabolism; (4) lipid metabolism; (5) amino acid metabolism; (6) mineral and vitamin metabolism; (7) cellular redox homeostasis; (8) stress response; and (9) apoptosis. The results of our temporal proteomics analysis reveal continuous impairment of intestinal development in neonatal piglets with IUGR. The findings have important implications for understanding metabolic defects in the small intestine of IUGR neonates and are expected to provide new strategies to improve their survival and growth.