DETECTION OF N-ACETYLCYSTEINE, CYSTEINE AND THEIR DISULFIDES IN URINE BY LIQUID-CHROMATOGRAPHY WITH A DUAL-ELECTRODE AMPEROMETRIC DETECTOR
DETECTION OF N-ACETYLCYSTEINE, CYSTEINE AND THEIR DISULFIDES IN URINE BY LIQUID-CHROMATOGRAPHY WITH A DUAL-ELECTRODE AMPEROMETRIC DETECTOR
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DOI：
10.1016/0731-7085(90)80011-d
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发表时间：
1990-01-01
影响因子：
3.4
通讯作者：
LUNTE, CE
中科院分区：
文献类型：
--
作者：
STENKEN, JA;PUCKETT, DL;LUNTE, CE
本文报道了尿中N-乙酰半胱氨酸、半胱氨酸及其二硫化物的测定方法。硫醇和二硫化物分离的反相离子对色谱与辛基硫酸钠作为离子对试剂和检测的双电极安培检测器使用Au/Hg汞齐电极。硫醇和二硫化物都可以用这个系统检测。此外，二聚体和混合二硫化物可以单独检测。
A method for the determination of N-acetylcysteine, cysteine and their disulfides in urine is described. The thiols and disulfides are separated by reversed-phase ion-pair chromatography with octyl sodium sulfate as the ion-pairing reagent and detected with a dual-electrode amperometric detector using Au/Hg amalgam electrodes. Both the thiols and disulfides are detected with this system. In addition, dimers and mixed disulfides can be detected individually.