Enhanced genetically encoded voltage indicators advance their applications in neuroscience
Enhanced genetically encoded voltage indicators advance their applications in neuroscience
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DOI：
10.1016/j.cobme.2019.10.010
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发表时间：
2019-12-01
影响因子：
3.9
通讯作者：
Gong, Yiyang
中科院分区：
文献类型：
--
作者：
Beck, Connor;Zhang, Diming;Gong, Yiyang
遗传编码的电压指示器报告具有高时空分辨率的膜电压。最近广泛的努力，以提高GEVI的亮度，灵敏度和动力学已经增加了GEVI的信噪比性能超过十倍，并降低了他们的响应时间亚毫秒制度。这种能力已经扩大了GEVI在体外和体内以细胞分辨率测量神经群体的膜电压的能力，所有这些都是高速的。GEVI的高电压保真度和快速响应在多种神经科学应用中揭示了新的生理现象。这些应用预示着未来对电压活动的有针对性的研究，这些研究利用GEVI报告来自遗传靶向神经群体的快速动态的能力。
Genetically encoded voltage indicators report membrane voltage with high spatiotemporal resolution. Extensive recent efforts to improve the GEVIs' brightness, sensitivity, and kinetics have increased the GEVIs' signal-to-noise performance by over ten-fold and lowered their response time to the sub-millisecond regime. Such capabilities have broadened the GEVIs' ability to measure membrane voltage of neural populations at cellular resolution in vitro and in vivo, all at high speeds. The GEVIs' high voltage fidelity and fast response have revealed novel physiological phenomena in multiple neuroscientific applications. Such applications portend future targeted studies of voltage activity that take advantage of the GEVIs' ability to report rapid dynamics from genetically-targeted neural populations.