Developmental switch from GABA to glycine release in single central synaptic terminals
Developmental switch from GABA to glycine release in single central synaptic terminals
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DOI：
10.1038/nn1170
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发表时间：
2004-01-01
影响因子：
25
通讯作者：
Ishibashi, H
中科院分区：
文献类型：
--
作者：
Nabekura, J;Katsurabayashi, S;Ishibashi, H
在出生后发育的早期，大鼠外侧上级橄榄（LSO）神经元的抑制性输入从主要释放GABA到主要释放甘氨酸进入突触。在这里，我们发现，自发的微型抑制性突触后电流（mIPSC）也从GABA能的甘氨酸能在出生后的前两周。在这种转变过程中，可以看到许多由甘氨酸和GABA从相同囊泡中共同释放而产生的“混合”mIPSC。免疫组化结果显示，在出生后第8天（P8），大量的终末含有GABA和甘氨酸。到P14时，这些混合终末中的GABA含量以及GABA对混合mIPSC的贡献均降低。在同一时期，末端中甘氨酸的含量增加。我们的研究结果表明，从GABA能到甘氨酸能输入LSO的转换可能发生在单个突触前末梢的水平。这证明了一种新形式的发展可塑性在一个单一的中央突触的水平。
Early in postnatal development, inhibitory inputs to rat lateral superior olive (LSO) neurons change from releasing predominantly GABA to releasing predominantly glycine into the synapse. Here we show that spontaneous miniature inhibitory postsynaptic currents (mIPSCs) also change from GABAergic to glycinergic over the first two postnatal weeks. Many 'mixed' mIPSCs, resulting from co-release of glycine and GABA from the same vesicles, are seen during this transition. Immunohistochemistry showed that a large number of terminals contained both GABA and glycine at postnatal day 8 (P8). By P14, both the content of GABA in these mixed terminals and the contribution of GABA to the mixed mIPSCs had decreased. The content of glycine in terminals increased over the same period. Our results indicate that switching from GABAergic to glycinergic inputs to the LSO may occur at the level of a single presynaptic terminal. This demonstrates a new form of developmental plasticity at the level of a single central synapse.