Preliminary comparison of plasma notch-associated microRNA-34b and-34c levels in drug naive, first episode depressed patients and healthy controls
Preliminary comparison of plasma notch-associated microRNA-34b and-34c levels in drug naive, first episode depressed patients and healthy controls
复制标题
初治首次发作抑郁症患者和健康对照者血浆缺口相关 microRNA-34b 和-34c 水平的初步比较
DOI：
10.1016/j.jad.2016.01.017
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发表时间：
2016
影响因子：
6.6
通讯作者：
Zhang Kerang
中科院分区：
文献类型：
--
作者：
Sun Ning;Lei Lei;Wang Yanfang;Yang Chunxia;Liu Zhifen;Li Xinrong;Zhang Kerang
重度抑郁症（MDD）是一种常见的致残性疾病，病因不明。miRNA的表达与抑郁症及抗抑郁治疗的疗效密切相关。然而，notch相关的mirna表达是否与首发MDD有关尚不清楚。方法采用qRT-PCR方法检测32例MDD患者和32例健康对照者外周血白细胞中Notch1、Hes1 mRNA及5种mirna （miR-369-3p、miR-34b-5p、miR-34c-5p、miR-381和miR-107）的表达水平。我们还评估了抑郁症状的严重程度、自杀风险水平、消极生活事件和事件相关电位P300。结果MDD患者miR-34b-5p（病例组中位数为62.49，对照组中位数为38.62）和miR-34c-5p（病例组中位数为7.17，对照组中位数为5.45）的表达水平显著高于对照组。notch1基因在MDD患者中显著降低（病例组中位数为5.35，对照组中位数为6.80），且与miR-34c-5p、miR-34b-5p的表达呈负相关。有自杀念头患者miR-34b-5p、miR-369-3p表达水平明显降低。P300的N1潜伏期与miR-34c-5p、miR-107、miR-381呈正相关，P300的P2潜伏期与miR-34c-5p、miR-107、miR-381呈正相关。局限性：样本量较小，候选mirna在Notch1基因调控和认知功能中的作用有待进一步研究。结论外周血notch相关mirna的差异表达可能与MDD有关，外周血白细胞miR-34b-5p和miR-34c-5p水平与MDD、自杀念头和认知功能密切相关，需要进一步大样本研究来验证这些mirna作为MDD生物标志物的可行性。
BackgroundMajor depressive disorder (MDD) is a common debilitating disease of unknown etiology. The expression of miRNA is closely related to depression and efficacy of antidepressant therapy. However, whether Notch-associated miRNAs expressions involved in first-episode of MDD are still unknown.MethodsIn this study, the expression levels of Notch1, Hes1 mRNA and 5 miRNAs (miR-369–3p, miR-34b–5p, miR-34c–5p, miR-381 and miR-107) in peripheral blood leukocytes of 32 MDD patients and 32 healthy controls were detected using qRT-PCR method. We also assessed the severity of depressive symptom, suicide risk level, negative life events and event-related potential P300.ResultsThe expression levels of miR-34b–5p (62.49 as the median of cases group and 38.62 as median of control group) and miR-34c–5p (7.17 as the median of cases group and 5.45 as median of control group) in MDD patients were significantly higher than these in control subjects.NOTCH1gene were significantly lower in MDD patients (5.35 as the median of cases group and 6.80 as median of control group), and was negatively correlated with the expression miR-34c-5p and miR-34b-5p. The expression level of miR-34b–5p and miR-369–3p were significantly lower in patients with suicide idea. N1 latency of P300 were positive correlated with miR-34c–5p, miR-107 and miR-381, and P2 latency of P300 were positive correlated with miR-34c–5p, miR-107 and miR-381.LimitationsThe sample size was small and the role of candidate miRNAs in the regulation of Notch1 gene and cognitive function are still need to be further investigated.ConclusionsDifferentially Notch-associated miRNAs expressions in peripheral blood might be involved in MDD, and the miR-34b–5p and miR-34c–5p levels in peripheral blood leukocytes are closely related to MDD, suicide idea and cognitive function, further studies with large sample size are warranted to test the feasibility of these miRNAs serving as biomarkers for MDD.