THE ISOLATION AND CHARACTERIZATION OF MUTANTS OF HERPES-SIMPLEX VIRUS TYPE-1 THAT INDUCE CELL-FUSION
THE ISOLATION AND CHARACTERIZATION OF MUTANTS OF HERPES-SIMPLEX VIRUS TYPE-1 THAT INDUCE CELL-FUSION
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DOI：
10.1099/0022-1317-61-2-245
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发表时间：
1982-01-01
影响因子：
3.8
通讯作者：
WARNER, SC
中科院分区：
文献类型：
--
作者：
BOND, VC;PERSON, S;WARNER, SC
从单纯疱疹病毒1型（HSV-1）的KOS毒株（syn-101 ~ syn-106）和HFEM毒株（syn-107 ~ syn-109）中分离到6个引起细胞融合的syn突变体。将这些突变体与KOS合胞体突变体syn-20和HFEM合胞体突变体ts-B5进行互补重组。一些研究还使用了从非合胞亲本MP中分离出来的合胞诱导菌株MP。syn-20和ts-B5的互补和重组表明，这两个突变体在两种不同的病毒基因中发生了改变。syn-20和ts-B5之间的重组频率与MP和ts-B5之间的重组频率非常相似，表明syn-20和MP可能代表同一病毒基因的改变。Syn-101、syn-103、syn-104和syn-105暂定归属于syn-20互补组，syn-107和syn-109暂定归属于ts-B5互补组。Syn-106和syn-108被排除在ts-B5组之外。Syn-102不能从两个互补基团中排除。Syn-101在38度时诱导的融合明显减少。C相对于34度。C. 34度。C经十二烷基硫酸钠凝胶电泳检测，syn-101感染的人胚胎肺细胞多肽和糖肽的模式正常，但温度为38度。C，糖肽gC的量明显减少。Syn-102产生的糖蛋白数量减少，并且在被syn-106感染的提取物中缺少非糖基化肽，可能是ICP6。
Six cell fusion-causing syn mutants were isolated from the KOS (syn-101 to syn-106) and 3 from the HFEM (syn-107 to syn-109) strains of herpes simplex virus type 1 (HSV-1). The mutants were studied by complementation and recombination with syn-20 (a syncytial mutant of KOS) and ts-B5 (a syncytial mutant of HFEM). Some studies also employed MP, a syncytium-inducing strain isolated from the non-syncytial parent, mP. Complementation and recombinatin of syn-20 and ts-B5 indicated that these 2 mutants were altered in 2 different virus genes. The recombination frequency between syn-20 and ts-B5 was very similar to that observed between MP and ts-B5, indicating that syn-20 and MP may represent alterations in the same virus gene. Syn-101, syn-103, syn-104 and syn-105 were tentatively assigned to the syn-20 complementation group, while syn-107 and syn-109 were tentatively assigned to the ts-B5 complementation group. Syn-106 and syn-108 were excluded from the ts-B5 group. Syn-102 could not be excluded from either complementation group. Syn-101 induced markedly less fusion at 38.degree. C relative to 34.degree. C. At 34.degree. C the patterns of syn-101-infected human embryonic lung cell peptides and glycopeptides, examined by sodium dodecyl sulfate gel electrophoresis, were normal, but at 38.degree. C the amount of glycopeptide gC was particularly reduced. Syn-102 produced decreased amounts of glycoproteins, and a non-glycosylated peptide, probably ICP6, was absent from extracts infected with syn-106.